M6018

4-Methylumbelliferyl β-D-cellobioside

Name: 4-Methylumbelliferyl &#946;-<SC>D</SC>-cellobioside
Brand: SIGMA

glucanase substrate, fluorogenic, ≥98.00% (TLC), powder

Synonym(s):

4-Methylumbelliferyl beta-D-cellobioside

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About This Item

Empirical Formula (Hill Notation):

C₂₂H₂₈O₁₃

CAS Number:

72626-61-0

Molecular Weight:

500.45

NACRES:

NA.32

PubChem Substance ID:

24897067

UNSPSC Code:

12352204

EC Number:

276-744-7

MDL number:

MFCD00063279

Key Documents

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Product Name

4-Methylumbelliferyl β-D-cellobioside, glucanase substrate

InChI key

PRTGXBPFDYMIJH-MKQZUAMYSA-N

InChI

1S/C22H28O13/c1-8-4-14(25)32-11-5-9(2-3-10(8)11)31-21-19(30)17(28)20(13(7-24)34-21)35-22-18(29)16(27)15(26)12(6-23)33-22/h2-5,12-13,15-24,26-30H,6-7H2,1H3/t12-,13-,15-,16+,17-,18-,19-,20-,21-,22+/m1/s1

SMILES string

CC1=CC(=O)Oc2cc(O[C@@H]3O[C@H](CO)[C@@H](O[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)ccc12

assay

≥98.00% (TLC)

form

powder

solubility

water: 19.60-20.40 mg/mL, clear, colorless to faintly yellow

storage temp.

2-8°C

Quality Level

200

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Show Differences

1 of 4

This Item	M9763	M2405	M0905
Sigma-Aldrich M6018 4-Methylumbelliferyl β-D-cellobioside	Sigma-Aldrich M9763 4-Methylumbelliferyl β-D-N,N′-diacetylchitobioside hydrate	Sigma-Aldrich M2405 4-Methylumbelliferyl β-D-lactopyranoside	Sigma-Aldrich M0905 4-Methylumbelliferyl β-D-mannopyranoside
assay ≥98.00% (TLC)	assay ≥98% (TLC)	assay ≥98.00% (TLC)	assay ≥98% (TLC)
solubility water: 19.60-20.40 mg/mL, clear, colorless to faintly yellow	solubility DMF: 9.80-10.20 mg/mL, clear, colorless to light yellow	solubility water: 9.80-10.20 mg/mL, clear, colorless to faintly yellow	solubility pyridine: 10 mg/mL, clear, colorless to faintly yellow
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 300
form powder	form powder	form powder	form powder
storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

Application

4-Methylumbelliferyl β-D-cellobioside has been used as a substrate:

to assay cellulase from A. niger[1]
to determine the extracellular enzyme activity for 1,4-β-cellobiohydrolase[2]
to assay the activity of bound enzyme, endoglucanase I (EGIc) Trichoderma reesei class II hydrophobins (HFBI)[3]

Biochem/physiol Actions

4-Methylumbelliferyl β-D-cellobioside is used as a substrate for cellulase assays. It is mainly used for activity staining of cellulases in polyacrylamide gels.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000453971

0000430619

0000401301

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Methylxanthine Drugs Are Chitinase Inhibitors: Investigation of Inhibition and Binding Modes

Rao FV, et al.

Chemistry & Biology, 12(9), 973-980 (2005)

A novel polysaccharide hydrolase cDNA (celD) from Neocallimastix patriciarum encoding three multi-functional catalytic domains with high endoglucanase, cellobiohydrolase and xylanase activities.

G P Xue et al.

Journal of general microbiology, 138(11), 2397-2403 (1992-11-01)

A plant polysaccharide hydrolase cDNA, designated celD, was isolated from a cDNA library of the rumen fungus Neocallimastix patriciarum. The enzyme encoded by celD had endoglucanase, cellobiohydrolase and xylanase activities. Deletion analysis revealed that celD cDNA can be truncated to

Reconciling opposing soil processes in row-crop agroecosystems via soil functional zone management

Williams A, et al.

Agriculture, Ecosystems & Environment, 236, 99-107 (2017)

The bifunctional enzyme chitosanase-cellulase produced by the gram-negative microorganism Myxobacter sp. AL-1 is highly similar to Bacillus subtilis endoglucanases.

M Pedraza-Reyes et al.

Archives of microbiology, 168(4), 321-327 (1997-09-23)

The gram-negative bacterium Myxobacter sp. AL-1 produces chitosanase-cellulase activity that is maximally excreted during the stationary phase of growth. Carboxymethylcellulase zymogram analysis revealed that the enzymatic activity was correlated with two bands of 32 and 35 kDa. Ion-exchange-chromatography-enriched preparations of

Cloning and expression of a Clostridium thermocellum xylanase gene in Escherichia coli.

K H Jung et al.

Biochemistry and molecular biology international, 44(2), 283-292 (1998-04-08)

A Clostridium thermocellum xylanase gene, designated xynX, was cloned in Escherichia coli and was categorized a novel gene as a result of the comparison of restriction patterns of the C. thermocellum xylanase genes so far reported. The xynX gene encodes

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