M9, Minimal Salts, 5X

powder, minimal microbial growth medium

M9 media

Quality Level


for molecular biology






KH2PO4, 15 g/L
NaCl, 2.5 g/L
Na2HPO4, 33.9 g/L
NH4Cl, 5 g/L

Featured Industry

Food and Beverages

storage temp.

room temp


nonselective for Escherichia coli
nonselective for coliforms

Related Categories

General description

M9 Minimal Salts is a highly-referenced microbial growth medium used for the cultivation of E. coli. This buffered minimal microbial medium contains only salts and nitrogen, so it is traditionally supplemented with glucose, amino acids and vitamins as needed.


M9, Minimal Salts, 5X has been used:
  • in a selective agar medium for the isolation of sulphate reducing bacteria
  • in GMML minimal media to propagate phage-resistant DH10B cells
  • in M9 minimal medium which is used for cell motility assay
M9 Minimal Salts 5x are suitable for the preparation of M9 Minimal Medium for use in propagation of E. coli and plasmid amplification. It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for maintaining positive selection pressure on E. coli that produce essential amino acids and for maintenance of F′-containing bacteria strains for M13 propagation.


1 kg in poly bottle

Features and Benefits

M9 powder provides:
  • Standard formulation
  • A budget-friendly alternative to liquid

Preparation Note

1. Dissolve 56.4 g in 1L of distilled water.
2. Autoclave for 15 minutes at 121°C.
This convenient 5× concentrate can be stored and diluted as needed to prepare 5 L of 1× M9 minimal salts.

For M9 minimal medium:
1. Aseptically dilute 200 ml of M9 minimal salts, 5× concentrate with 800 ml of sterile water. If necessary, cool to 45-50°C.
2. Aseptically add 20 ml of sterile 1 M glucose and 2 ml of sterile 1 M magnesium sulfate to prepare 1 L of M9 minimal medium.
3. If desired, aseptically add 0.1 ml of 1 M sterile calcium chloride to the M9 minimal medium. The M9 minimal medium may also be supplemented with the appropriate amino acids.


Make a 5x concentrated stock solution by stirring to suspend 56.4g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Dilute the 5x M9 concentrated stock to a 1x working solution by adding 200mL 5x M9 stock to 800mL sterile water. If needed, allow to cool before making additions, such as antibiotics (if desired).

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Matthew J Culyba et al.
PLoS genetics, 14(6), e1007405-e1007405 (2018-06-02)
Biochemical pathways are often genetically encoded as simple transcription regulation networks, where one transcription factor regulates the expression of multiple genes in a pathway. The relative timing of each promoter's activation and shut-off within the network can impact physiology. In...
Intestinimonas butyriciproducens gen. nov., sp. nov., a butyrate-producing bacterium from the mouse intestine.
Klaring K
International Journal of Systematic and Evolutionary Microbiology, 63, 4606-4612 (2013)
David Chen et al.
Molecular systems biology, 8, 620-620 (2012-10-24)
Natural biological systems have evolved a diverse array of switches to realize their strategies for environmental response and development. Emerging applications of synthetic biology have begun to exploit such switches to achieve increasingly sophisticated designed behaviors. However, not all switch...
An expanded genetic code with a functional quadruplet codon.
Anderson JC
Proceedings of the National Academy of Sciences of the USA, 101(20) (2004)
Lauren B A Woodruff et al.
Nucleic acids research, 45(3), 1553-1565 (2016-12-23)
Genetic designs can consist of dozens of genes and hundreds of genetic parts. After evaluating a design, it is desirable to implement changes without the cost and burden of starting the construction process from scratch. Here, we report a two-step...
General protocols for growth of competent cells in microbial medium.
Read More

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