When autoclaved or filter sterilized, the prepared solution may be stored tightly sealed at room temperature for up to 2 years.
M6030
M9, Minimal Salts, 5X
powder, minimal microbial growth medium
Synonym(s):
M9 media
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1 KG
$292.00
$292.00
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About This Item
UNSPSC Code:
12352302
NACRES:
NA.85
Grade:
Molecular Biology
Form:
powder
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grade
Molecular Biology
Quality Level
sterility
non-sterile
form
powder
composition
KH2PO4, 15 g/L
NaCl, 2.5 g/L
Na2HPO4, 33.9 g/L
NH4Cl, 5 g/L
application(s)
food and beverages
storage temp.
room temp
suitability
nonselective for Escherichia coli
nonselective for coliforms
1 of 4
This Item | M9956 | Y2377 | L3272 |
|---|---|---|---|
| form powder | form liquid | form powder | form powder |
| Quality Level 200 | Quality Level 500 | Quality Level 200 | Quality Level 200 |
| grade for molecular biology | grade for molecular biology | grade for molecular biology | grade for molecular biology |
| sterility non-sterile | sterility sterile; 0.2 μm filtered | sterility non-sterile | sterility non-sterile |
| composition KH2PO4, 15 g/L | composition - | composition - | composition Agar, 15 g/L , NaCl, 0.5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L |
| application(s) food and beverages | application(s) - | application(s) food and beverages | application(s) food and beverages |
General description
M9 Minimal Salts is a highly-referenced microbial growth medium used for the cultivation of E. coli. This buffered minimal microbial medium contains only salts and nitrogen, so it is traditionally supplemented with glucose, amino acids and vitamins as needed.
Application
M9, Minimal Salts, 5X has been used:
M9 Minimal Salts 5x are suitable for the preparation of M9 Minimal Medium for use in propagation of E. coli and plasmid amplification. It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for maintaining positive selection pressure on E. coli that produce essential amino acids and for maintenance of F′-containing bacteria strains for M13 propagation.
M9 Minimal Salts 5x are suitable for the preparation of M9 Minimal Medium for use in propagation of E. coli and plasmid amplification. It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for maintaining positive selection pressure on E. coli that produce essential amino acids and for maintenance of F′-containing bacteria strains for M13 propagation.
Features and Benefits
M9 powder provides:
- Standard formulation
- A budget-friendly alternative to liquid
Preparation Note
1. Dissolve 56.4 g in 1L of distilled water.
2. Autoclave for 15 minutes at 121°C.
This convenient 5× concentrate can be stored and diluted as needed to prepare 5 L of 1× M9 minimal salts.
For M9 minimal medium:
1. Aseptically dilute 200 ml of M9 minimal salts, 5× concentrate with 800 ml of sterile water. If necessary, cool to 45-50°C.
2. Aseptically add 20 ml of sterile 1 M glucose and 2 ml of sterile 1 M magnesium sulfate to prepare 1 L of M9 minimal medium.
3. If desired, aseptically add 0.1 ml of 1 M sterile calcium chloride to the M9 minimal medium. The M9 minimal medium may also be supplemented with the appropriate amino acids.
2. Autoclave for 15 minutes at 121°C.
This convenient 5× concentrate can be stored and diluted as needed to prepare 5 L of 1× M9 minimal salts.
For M9 minimal medium:
1. Aseptically dilute 200 ml of M9 minimal salts, 5× concentrate with 800 ml of sterile water. If necessary, cool to 45-50°C.
2. Aseptically add 20 ml of sterile 1 M glucose and 2 ml of sterile 1 M magnesium sulfate to prepare 1 L of M9 minimal medium.
3. If desired, aseptically add 0.1 ml of 1 M sterile calcium chloride to the M9 minimal medium. The M9 minimal medium may also be supplemented with the appropriate amino acids.
Make a 5x concentrated stock solution by stirring to suspend 56.4g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Dilute the 5x M9 concentrated stock to a 1x working solution by adding 200mL 5x M9 stock to 800mL sterile water. If needed, allow to cool before making additions, such as antibiotics (if desired).
Dilute the 5x M9 concentrated stock to a 1x working solution by adding 200mL 5x M9 stock to 800mL sterile water. If needed, allow to cool before making additions, such as antibiotics (if desired).
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Protocols
General protocols for growth of competent cells in microbial medium.
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Where does the 5x concentrate need to be stored? At room temperature or at 4°C? And how long would the shelf life be?
1 answer-
Helpful?
-
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What is the preparation ratio for the culture medium?
1 answer-
The preparation instructions are listed on the product detail page in the DESCRIPTION section under 'Preparation'.
Please navigate to the link below:
https://www.sigmaaldrich.com/product/sigma/m60301. Dissolve 56.4 g in 1L of distilled water.
2. Autoclave for 15 minutes at 121°C.
This convenient 5X concentrate can be stored and diluted as needed to prepare 5 L of 1X M9 minimal salts.For M9 minimal medium:
1. Aseptically dilute 200 mL of M9 minimal salts, 5X concentrate with 800 mL of sterile water. If necessary, cool to 45-50°C.
2. Aseptically add 20 mL of sterile 1 M glucose and 2 mL of sterile 1 M magnesium sulfate to prepare 1 L of M9 minimal medium.
3. If desired, aseptically add 0.1 mL of 1 M sterile calcium chloride to the M9 minimal medium. The M9 minimal medium may also be supplemented with the appropriate amino acids.Helpful?
-
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