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M6159

Sigma-Aldrich

α2-Macroglobulin from human plasma

BioUltra, ≥98% (SDS-PAGE)

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Synonym(s):
α2-M
CAS Number:
MDL number:

biological source

human plasma

Quality Level

product line

BioUltra

assay

≥98% (SDS-PAGE)

form

lyophilized powder

mol wt

~720 kDa (four glycoprotein subunits)

composition

Protein, 15-30% biuret

technique(s)

inhibition assay: suitable

solubility

water: soluble 10 mg protein/mL, clear, colorless

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... A2M(2)

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This Item
63013A9024SRP6314
α2-Macroglobulin from human plasma BioUltra, ≥98% (SDS-PAGE)

M6159

α2-Macroglobulin from human plasma

α2-Macroglobulin from human plasma ≥90% (GE)

63013

α2-Macroglobulin from human plasma

α1-Antitrypsin from human plasma salt-free, lyophilized powder

A9024

α1-Antitrypsin from human plasma

biological source

human plasma

biological source

human plasma

biological source

human plasma

biological source

human

Gene Information

human ... A2M(2)

Gene Information

human ... A2M(2)

Gene Information

human ... ELA2(1991), SERPINA1(5265)

Gene Information

human ... A2M(2)

Quality Level

200

Quality Level

100

Quality Level

-

Quality Level

-

form

lyophilized powder

form

-

form

salt-free, lyophilized powder

form

lyophilized

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Application

α2-Macroglobulin was used to study impaired lipoprotein receptor-mediated peripheral binding of plasma amyloid-β which is an early biomarker for mild cognitive impairment preceding Alzheimer′s disease. It was also used in the control of the classical and the MBL pathway of complement activation.
Inhibits all classes of endoproteases by forming a complex with the protease. When the protease cleaves the macroglobulin "bait" sequence, the macroglobulin rearranges and traps the protease.

Biochem/physiol Actions

α2-Macroglobulin (α2M) is a multifunctional protein that is a broad spectrum protease inhibitor. α2M is a large homotetrameric glycoprotein (720 kDa) that is connected by disulfide-linked dimers which non-covalently interact to give the quaternary structure. α2M is found in normal plasma at a concentration of 220-230 mg/dl accounting for 3-5% of the total plasma protein.
α2-Macroglobulin is found abundantly in plasma and interstitial fluids. The protease-α2-M balance plays an important role in mediating inflammatory tissue destruction. Serum levels of α2-M and protease-α2-M complexes are increased in patients with sepsis, emphysema, periodontitis, rheumatoid arthritis, and other inflammatory diseases, and oxidant inactivation of α2-M may contribute to tissue destruction during inflammation.

Packaging

Package size based on protein content

Physical form

Lyophilized from 0.02 M Tris, 0.13 M glycine, pH 8.0, and 0.08 M trehalose

Analysis Note

Plasma from each donor has been tested and found negative for antibody to HIV-1/HIV-2, antibody to HCV and HbSAg.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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