M6518

Sigma-Aldrich

Macrophage Colony-Stimulating Factor human

M-CSF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):
MCSF
MDL number:
NACRES:
NA.77

Quality Level

biological source

human

recombinant

expressed in E. coli

assay

≥98% (SDS-PAGE and HPLC)

form

lyophilized powder

potency

0.500-5.000 ng/mL ED50

quality

endotoxin tested

mol wt

dimer 37 kDa

packaging

pkg of 10 μg

application(s)

cell culture | mammalian: suitable

impurities

≤1.000 EU/μg

color

white

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... CSF1(1435)

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General description

Macrophage Colony-Stimulating Factor (M-CSF) is a hematopoietic growth factor, which modulates the proliferation, differentiation and functional activation of monocytes. It is produced by fibroblasts, endothelial cells and bone marrow stromal cells. MCSF stimulates the functions of mature monocytes and macrophages.

Application

Macrophage Colony-Stimulating Factor human has been used for the differentiation of bone marrow-derived macrophages (BMDMs) and human monocyte-derived macrophages (MDMs). It has also been used for cell culture.

Biochem/physiol Actions

Macrophage colony-stimulating factor (M-CSF) augments many of the physiological functions of macrophages, and support osteoclast differentiation.

Other Notes

159 amino acids including N-terminal methionine (each monomer).

Physical form

Lyophilized from a sterile filtered buffered solution.

Analysis Note

The proliferative activity is tested in culture using the cytokine-dependent murine monocytic cell line M-NFS-60 cells.

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Avery's Diseases of the Newborn (2012)
Gianluca Ferlazzo et al.
Frontiers in veterinary science, 7, 335-335 (2020-08-08)
Porcine reproductive and respiratory syndrome (PRRS) affects farmed swine causing heavy direct and indirect losses. The infections sustained by PRRS viruses (PRRSV-1 and PRRSV-2) may give rise to severe clinical cases. This highlights the issue of PRRSV pathogenicity and relevant...
Denise Buenrostro et al.
Bone, 113, 77-88 (2018-05-14)
The tumor-cell microenvironment is recognized as a dynamic place where critical cell interactions occur and play an important role in altering tumorigenesis. While many studies have investigated the effects of cellular cross-talk within distinct tumor microenvironments, these interactions have yet...
Encyclopedia of Immunology (1998)
MIR144 inhibits antimicrobial responses against Mycobacterium tuberculosis in human monocytes and macrophages by targeting the autophagy protein DRAM2
Kim JK, et al.
Autophagy, 13(2), 423-441 (2017)
Articles
Read article on hematopoietic cytokines and hematopoiesis
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