M6693
Monoclonal Anti-MAT-Tag® antibody produced in mouse
purified immunoglobulin, ~2 mg/mL, clone MAT1-87
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
MAT1-87, monoclonal
concentration
~2 mg/mL
technique(s)
immunoprecipitation (IP): 5-10 μg
western blot: 1-2 μg/mL
isotype
IgG2a
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Related Categories
1 of 4
This Item | R5404 | R8529 | P3118 |
|---|---|---|---|
| clone MAT1-87, monoclonal | clone Rab9-29, monoclonal | clone RalA5F9.2, monoclonal | clone 5D6, monoclonal |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin |
| isotype IgG2a | isotype IgG1 | isotype IgG1 | isotype IgG1 |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
General description
Monoclonal Anti-MAT (mouse IgG2a isotype) is derived from the MAT1-87 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic MAT peptide HNHRHKHGGGC conjugated to KLH.
Monoclonal Anti-Mat-Tag® antibody was used in one-step purification of histidine-tagged fusion proteins under high pressure and high flow conditions. It is used in the characterization of the molecular architecture of human caveolin-3 and interaction with the skeletal muscle ryanodine receptor.
Immunogen
synthetic MAT peptide HNHRHKHGGGC.
Application
Monoclonal Anti-MAT-Tag® antibody produced in mouse has been used in:
- enzyme linked immunosorbent assay (ELISA)
- immunoblotting[1]
- immunoprecipitation
- immunocytochemistry
The MAT tag is a seven amino acid peptide tag that binds to transition metals such as Nickel and Cobalt. This tag, which consists of the sequence HNHRHKH, allows the purification of MAT fusion proteins using immobilized metal affinity chromatography (IMAC) such as the highly selective HIS-Select® Nickel Affinity Gel. The MAT tag sequence can be incorporated into both prokaryotic and eukaryotic expression vectors.
Biochem/physiol Actions
Monoclonal Anti-MAT recognizes the seven amino acid MAT epitope sequence within either N or C terminal MAT-tagged fusion proteins.
Physical form
Supplied as a solution in 0.01M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Legal Information
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
MAT-Tag is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Caveolin-3 facilitates both caveolae formation and a range of cell signaling pathways, including Ca(2+) homeostasis. Caveolin-3 forms a disc-shaped nonamer that binds the Ca(2+)-release channel, RyR1. Multiple caveolin-3 nonamers bind to a single RyR1 homotetramer. First three-dimensional structural insights into
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