MilliporeSigma
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M6818

Sigma-Aldrich

Anti-MeCP2 antibody, Mouse monoclonal

clone Mec-168, purified from hybridoma cell culture

Synonym(s):
Anti-AUTSX3, Anti-MRX16, Anti-MRX79, Anti-MRXS13, Anti-MRXSL, Anti-PPMX, Anti-RS, Anti-RTS, Anti-RTT
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

Mec-168, monoclonal

form

buffered aqueous solution

mol wt

antigen ~75 kDa

species reactivity

rat, human, mouse

packaging

antibody small pack of 25 μL

technique(s)

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.1-0.2 μg/mL using nuclear cell extract of cultured Jurkat, human acute T cell leukemia cells, or cultured MCF7 human breast carcinoma.

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... MECP2(4204)
mouse ... Mecp2(17257)
rat ... Mecp2(29386)

Related Categories

General description

Monoclonal Anti-MeCP2 (mouse IgG1 isotype) is derived from the Mec-168 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the C- terminus of human MeCP2. Methyl-CpG binding protein 2 (MeCP2) is the first methyl-CpG-binding protein to be isolated. This protein contains a methyl-CpG-binding domain (MBD) and a transcriptional repression domain (TRD).[1]

Immunogen

synthetic peptide corresponding to the C-terminus (amino acids 471-486) of human MeCP2.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
Western Blotting (1 paper)
Monoclonal Anti-MeCP2 antibody produced in mouse has been used in:
  • immunoblotting[2][3]
  • immunoprecipitation[2][3]
  • immunostaining[4]
  • enzyme linked immunosorbent assay (ELISA)
  • immunocytochemistry
  • western blot (0.1-0.2 μg/mL using nuclear cell extract of cultured Jurkat, human acute T cell leukemia cells, or cultured MCF7 human breast carcinoma)

Biochem/physiol Actions

Methyl-CpG binding protein 2 (MeCP2) is a transcription modulator that binds methylated DNA. This protein regulates neuronal functions and central nervous system development. Alterations in MeCP2 have been associated with neurological diseases such as MECP2 duplication syndrome and Rett syndrome [5][6]
Methyl-CpG binding protein 2 (MeCP2) deacetylase (HDAC) repressive machinery via recruitment of the Sin 3A corepressor, thus removing acetyl groups from histones and consequently, silencing genes.[1] Antibodies reacting specifically with MeCP2 may be used for studying chromatin remodeling effects on gene expression.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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