Monoclonal Anti-MeCP2 (mouse IgG1 isotype) is derived from the Mec-168 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the C- terminus of human MeCP2. Methyl-CpG binding protein 2 (MeCP2) is the first methyl-CpG-binding protein to be isolated. This protein contains a methyl-CpG-binding domain (MBD) and a transcriptional repression domain (TRD).
synthetic peptide corresponding to the C-terminus (amino acids 471-486) of human MeCP2.
Monoclonal Anti-MeCP2 antibody produced in mouse has been used in:
- enzyme linked immunosorbent assay (ELISA)
- western blot (0.1-0.2 μg/mL using nuclear cell extract of cultured Jurkat, human acute T cell leukemia cells, or cultured MCF7 human breast carcinoma)
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
Western Blotting (1 paper)
Methyl-CpG binding protein 2 (MeCP2) is a transcription modulator that binds methylated DNA. This protein regulates neuronal functions and central nervous system development. Alterations in MeCP2 have been associated with neurological diseases such as MECP2 duplication syndrome and Rett syndrome
Methyl-CpG binding protein 2 (MeCP2) deacetylase (HDAC) repressive machinery via recruitment of the Sin 3A corepressor, thus removing acetyl groups from histones and consequently, silencing genes. Antibodies reacting specifically with MeCP2 may be used for studying chromatin remodeling effects on gene expression.
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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