The MEM vitamin solution is a published classical formulation containing numerous components. See the link below to review the formulation of this and other classical vitamin and amino acid mixtures:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/167/485/m6895for.pdf
M6895
MEM Vitamin Solution (100×)
sterile-filtered, BioReagent, suitable for cell culture
Synonym(s):
MEM media, MEM vitamin solution
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100 ML
$27.70
About This Item
UNSPSC Code:
12352205
eCl@ss:
32160406
NACRES:
NA.75
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sterility
sterile-filtered
Quality Level
product line
BioReagent
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
color
yellow to dark yellow
pH
7.0-8.0
application(s)
agriculture
shipped in
dry ice
storage temp.
−20°C
1 of 4
This Item | B6891 | R7256 | M7145 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| application(s) agriculture | application(s) - | application(s) - | application(s) - |
| form liquid | form liquid | form liquid | form liquid |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in - |
General description
MEM Vitamin Solution (100×) is a sterile-filtered bioreagent, which is suitable for cell culture.
Synthetic/ basal media used currently contain amino acids, carbohydrates, vitamins and salts. Eagle′s minimum essential medium (MEM) is a modified version of BME (basal medium Eagle′s), which contains increased amounts of each amino acid.[1] Addition of water soluble vitamins such as, inositol to various culture facilitates the hatching of hamster and rabbit blastocysts. Vitamins also influence the metabolism of glucose in sheep and mouse embryos.[2]
Application
MEM Vitamin Solution (100×) has been used in:
- glial conditioned medium for primary neuronal culture[3]
- in Luria-Bertani (LB) medium or minimal medium (MM)[4]
- in private medium (PM = aminoacid-starved medium) for the Aa deprivation experiments[5]
MEM Vitamin Solution (100×) is used for the following applications:
Adding supplements of vitamins to media stimulates growth and prolongs the viability of cells in culture. This concentrated supplement adds nutrients and reduces the biosynthetic burden on cell in vitro.
- In the preparation of DMEM medium (for primary neural cell culture)[6][3][7]
- For the maintenance of cell lines in Eagle′s Minimum Essential Medium (the medium is supplemented with MEM Vitamin Solution along with other components)[8]
Adding supplements of vitamins to media stimulates growth and prolongs the viability of cells in culture. This concentrated supplement adds nutrients and reduces the biosynthetic burden on cell in vitro.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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MEM ビタミン溶液（100X）の組成を教えていただきたいです。
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