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M6900

Sigma-Aldrich

Methyl Blue

powder or crystals

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Synonym(s):
Acid blue 93, Aniline blue water soluble, Cotton blue, Poirriers blue, Water blue
Empirical Formula (Hill Notation):
C37H27N3Na2O9S3
CAS Number:
Molecular Weight:
799.80
Colour Index Number:
42780
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

form

powder or crystals

mp

>-23 °C

solubility

H2O: 1 mg/mL, blue

εmax

≥650 at 594-610  nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].OS(=O)(=O)c1ccc(Nc2ccc(cc2)\C(c3ccc(Nc4ccc(cc4)S([O-])(=O)=O)cc3)=C5/C=CC(\C=C5)=N/c6ccc(cc6)S([O-])(=O)=O)cc1

InChI

1S/C37H29N3O9S3.2Na/c41-50(42,43)34-19-13-31(14-20-34)38-28-7-1-25(2-8-28)37(26-3-9-29(10-4-26)39-32-15-21-35(22-16-32)51(44,45)46)27-5-11-30(12-6-27)40-33-17-23-36(24-18-33)52(47,48)49;;/h1-24,38-39H,(H,41,42,43)(H,44,45,46)(H,47,48,49);;/q;2*+1/p-2

InChI key

MCPLVIGCWWTHFH-UHFFFAOYSA-L

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This Item
95290M5528302643
vibrant-m

M6900

Methyl Blue

vibrant-m

95290

Methyl Blue

vibrant-m

M5528

Methyl Blue

vibrant-m

302643

Trypan Blue

form

powder or crystals

form

powder or crystals

form

powder

form

powder

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

εmax

≥650 at 594-610  nm

εmax

≥200 at 310-320 nm, ≥400 at 595-605 nm

εmax

≥700 at 594-610 nm in 5 mM HCl

εmax

-

General description

Methyl Blue is a triaminotriphenylmethane dye, widely used in paper, textile and leather production.

Application

Methyl Blue has been used:
  • to stain leaf disks for callose visualization within the cells
  • to incubate cells for 4′,6-diamidino-2-phenylindole (DAPI)/septa staining
  • as a marker for β-(1-3)-d-glucans or acidic phloroglucinol for the detection of callose and lignin

Biochem/physiol Actions

Methyl Blue is considered as a xenobiotic compound and has antiseptic property. It is useful in polychrome staining. Methyl blue is mostly used for staining samples in histology and microbiology. It is also useful as a model to reveal the role of catalyst in light induced degradation of dyes.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 2

flash_point_f

572.0 °F - Pensky-Martens closed cup

flash_point_c

> 300 °C - Pensky-Martens closed cup

ppe

dust mask type N95 (US), Eyeshields, Gloves


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A novel a-1, 3-glucan elicits plant defense responses in potato and induces protection against Rhizoctonia solani AG-3 and Fusarium solani f. sp. eumartii.
Wolski E
Physiological & Molecular Plant Pathology, 69, 93-93 (2006)
Biodegradation of basic violet 3 and acid blue 93 by Pseudomonas putida
Khan SS, et al.
Clean: Soil, Air, Water, 43(1), 67-72 (2015)
Replication stress in early S phase generates apparent micronuclei and chromosome rearrangement in fission yeast
Sabatinos SA, et al.
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A new principle in polychrome staining: a system of automated staining, complementary to hematoxylin and eosin, and usable as a research tool
Shoobridge Michael PK
Stain Technology, 58(5), 245-258 (1983)
Micellar effect on the kinetics of oxidation of methyl blue by Ce (IV) in sulfuric acid medium
Hassan M, et al.
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