M6908

Myeloperoxidase from human leukocytes

lyophilized powder, ≥50 units/mg protein

• Synonym(s): MPO, Peroxidase, myelo
• CAS Number: 9003-99-0
• EC Number: 1.11.2.2 (BRENDA, IUBMB)
• MDL number: MFCD00131641
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human leucocytes
• Quality Level: 200
• form: lyophilized powder
• specific activity: ≥50 units/mg protein
• solubility: H₂O: soluble; aqueous buffers such as 50 mM sodium acetate: soluble (Freezing of the enzyme in solution may result in a substantial loss of activity.)
• UniProt accession no.: P05164
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: [O+H2]O[O-]
• InChI: 1S/H2O3/c1-3-2/h1-2H
• InChI key: JSPLKZUTYZBBKA-UHFFFAOYSA-N
• Gene Information: human ... MPO(4353)

Description

General description

Myeloperoxidase (MPO) is encoded by the gene mapped to human chromosome 17q22-24.[1] The encoded protein is a leukocyte-derived enzyme and is an integral constituent of the innate immune response. MPO belongs to the heme peroxidase superfamily. MPO is a heme protein containing iron and is highly expressed in leukocytes, neutrophils, monocytes and some subtypes of tissue macrophages.[2]

Application

Myeloperoxidase (MPO) from human leukocytes has been used in MPO assay.[3][4][5]

Myeloperoxidase from human leukocytes has been used:

• as a standard to quantify the neutrophil (polymorphonuclear cell [PMN]) infiltration in lung tissue[6]
• as a neutrophil extracelullar trap (NET) component to stimulate 10⁶ dendritic cells or 10⁶ macrophages[7]
• as a standard in myeloperoxidase (MPO) assay to measure pancreatic MPO[8]

Biochem/physiol Actions

Myeloperoxidase (MPO) has a crucial role to play in destruction of various microorganisms and foreign cells, such as bacteria, fungi, viruses, red cells and malignant and nonmalignant nucleated cells.[9] MPO catalyzes the production of number of reactive oxidant species (ROS) that influence tissue damage during inflammation. MPO and its downstream inflammatory pathways function as a potent therapeutic target for prophylaxis of atherosclerotic cardiovascular disease.[10] MPO catalyze the synthesis of polychlorinated dibenzo(p)dioxins and furans (PCDD/F) from precursor such as chlorophenols in presence of hydrogen peroxide (H₂O₂).[3]

Physical form

Lyophilized from 50 mM sodium acetate buffer, pH 6.0, 0.1 M sodium chloride

Analysis Note

Source material tested and found negative for antibody to HIV and for HBSAG.

Other Notes

Lysosomal heme-containing protein.

One unit of myeloperoxidase will catalyze the consumption of one micromole of hydrogen peroxide and the production of ¹/₄ micromole of tetraguaiacol per minute at pH 7.0 and 25 °C

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable