This product does not contain Agar. A link to the plant media formulations can be found in the DESCRIPTION section of the product page. Please see the link below:
https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/plant-tissue-culture/murashige-skoog
M7024
Murashige and Skoog Shoot Multiplication Medium A
powder, suitable for plant cell culture
Synonym(s):
MSMA
Skip To
form
powder
Quality Level
technique(s)
cell culture | plant: suitable
application(s)
agriculture
shipped in
ambient
storage temp.
2-8°C
1 of 4
This Item | M9274 | M0404 | M5519 |
|---|---|---|---|
| application(s) agriculture | application(s) agriculture | application(s) agriculture | application(s) agriculture |
| technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable |
| form powder | form powder | form powder | form powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Application
Murashige and Skoog Shoot Multiplication Medium A is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).
Preparation Note
Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Shoot Multiplication Medium A (M7024 is a variant media) with macro- and micronutrients, vitamins, sucrose, IAA, sodium phosphate, adenine and 2iP.
With the macro- and micronutrients, vitamins, sucrose, IAA, sodium phosphate, adenine and 2iP as described by Huang and Murashige (1976) TCA Manual.
Media Formulation
Media Formulation
signalword
Warning
hcodes
Hazard Classifications
Ox. Sol. 3
Storage Class
5.1B - Oxidizing hazardous materials
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Regulation of lycopene formation in cell suspension culture of VFNT tomato (Lycopersicon esculentum) by CPTA, growth regulators, sucrose, and temperature
Robertson GE, Mahoney NE
Journal of Experimental Botany, 46, 667-673 (1995)
Plant tissue culture media: major constituents, their preparation and some applications.
Huang L, Murashige T.
Tissue Antigens, 3, 539-548 (1976)
Nor Azlina Hasbullah et al.
Pakistan journal of biological sciences : PJBS, 11(11), 1449-1454 (2008-09-27)
Regeneration potentials in Gerbera jamesonii Bolus ex. Hook f. from tissues culture system was studied using leaf, petiole and root explants. In vitro regeneration, callus induction and root formation were optimized by manipulation of growth regulators during organogenesis. Various kinds
R H Smith et al.
In vitro, 13(5), 329-334 (1977-05-01)
Defined in vitro conditions for callus initiation by Gossypium arboreum L. were determined, and different tissues were evaluated as explant sources, Environmental conditions tested included light versus dark, and low light versus high light. Different nutrient media as well as
Akasaka et al.
Plant science : an international journal of experimental plant biology, 156(2), 169-175 (2000-08-11)
Bud primordia were induced from leaf segments, which were harvested from young seedlings of Spanish type peanut (Arachis hypogaea L. cv. Chico), on 0.8% agar-solidified medium containing Murashige and Skoog (MS) basal salts supplemented with B5 vitamins, 1 mg/l NAA
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In the description of M7024, it is written that the medium, in addition to macro and micronutrients, contains sucrose, IAA and Kinetin. It is also described it contains agar, but the medium did not solidify. What percentage of agar in the product?
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