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M7167

Sigma-Aldrich

M2 medium

With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for mouse embryo cell culture

MDL number:
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

storage condition

protect from light

technique(s)

cell culture | embryo: suitable

impurities

endotoxin, tested

components

phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L
HEPES: 5.42726 g/L
glucose: 1.0 g/L (Dextro)
NaHCO3: 0.35 g/L

shipped in

ambient

storage temp.

2-8°C

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Application

Recommended for manipulation of mouse embryos at ambient temperature. M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently.

M2 Medium is a further modification of M16 that substitutes HEPES buffer in place of some of the bicarbonate. M2 is used for collecting and handling embryos for prolonged periods outside a CO2 incubator.

Quality

Presence of particulates in solution will not affect product′s performance.

Reconstitution

Supplement with 0.06 g/L potassium penicillin-G and 0.05 g/L streptomycin sulfate.

Analysis Note

This product is tested for its ability to support the development of one-cell mouse embryos to expanded blastocysts. Minimum requirement is 80% development to blastocyst.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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