M7257

α-Mannosidase from Canavalia ensiformis (Jack bean)

ammonium sulfate suspension, ≥15 units/mg protein (biuret)

• Synonym(s): α-D-Mannoside mannohydrolase
• CAS Number: 9025-42-7
• Enzyme Commission number: 3.2.1.24 (BRENDA, IUBMB)
• EC Number: 232-794-1
• MDL number: MFCD00130495
• NACRES: NA.54

Properties

• biological source: Canavalia ensiformis
• Quality Level: 200
• form: ammonium sulfate suspension
• specific activity: ≥15 units/mg protein (biuret)
• foreign activity: β-galactosidase, β-N-acetylglucosaminidase, α-galactosidase and α-L-fucosidase <0.05%
• storage temp.: 2-8°C
• InChI: 1S/Zn/q+2
• InChI key: PTFCDOFLOPIGGS-UHFFFAOYSA-N

Description

General description

α−Mannosidase from Canavalia ensiformis is a tretamer composed of two subunits that each contain two components at 44 and 66 kDa.

α-Mannosidase is an exo-glycosidase and an inc metalloenzyme.

Lysosomal α-mannosidases are class II mannosidases that belong to glycoside hydrolase family 38 and play an important role in the degradation of asparagine-linked carbohydrates of glycoproteins.

Application

α-Mannosidase can be used to liberate mannose from a variety of synthetic and natural α-mannosides. It has also been used in a study to investigate the causes of neurodegeneration in mucolipidosis II ′knock-in′ mice.

α-Mannosidase from Canavalia ensiformis (Jack bean) has been used:

• for the digestion of neutral glycans.
• to remove O-glycans from recombinant β-glucosidase
• for the inhibition of renal fibroblasts proliferation

Liberates mannose from a variety of synthetic and natural α-mannosides.

Biochem/physiol Actions

α−Mannosidase has been shown to inhibit the proliferation of B-lymphocytes.

α-Mannosidase cleaves near α1−2-, α1−3- and α1−6-linked mannose residues.

α-Mannosidase is an acid hydrolase which is located in plant vacuoles and is thought to be involved with the turnover of N-linked glycoproteins.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl α-D-mannoside to p-nitrophenol and D-mannose per min at pH 4.5 at 25°C.

Physical form

Suspension in 3.0 M (NH₄)₂SO₄ and 0.1 mM zinc acetate, pH 7.5

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves