All Photos(1)



Minimum Essential Medium Eagle

HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture


Quality Level






cell culture | mammalian: suitable


endotoxin, tested

pH range



L-glutamine: no
NaHCO3: yes
HEPES: 25 mM
phenol red: yes
Earle’s salts (5% CO2): yes
sodium pyruvate: no

shipped in


storage temp.


Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Minimum Essential Medium Eagle comprises sugar, inorganic salts, water-soluble vitamins and essential amino acids. It is widely used as a media formulation for many cell culture studies.


Minimum Essential Medium Eagle has been used for culturing primordial follicles and ovary tissues, human skin fibroblast cells, human colonic epithelial cell line LS174T and hippocampal tissue slices.


Supplement with 0.292 g/L L-glutamine.

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Johannes Vierock et al.
Nature communications, 12(1), 4527-4527 (2021-07-28)
Optogenetic manipulation of neuronal activity through excitatory and inhibitory opsins has become an indispensable experimental strategy in neuroscience research. For many applications bidirectional control of neuronal activity allowing both excitation and inhibition of the same neurons in a single experiment...
Rodrigo Lerchundi et al.
Frontiers in cellular neuroscience, 14, 80-80 (2020-05-07)
Genetically encoded nanosensors such as the FRET-based adenosine triphosphate (ATP) sensor ATeam enable the measurement of changes in ATP levels inside cells, promoting our understanding of metabolic interactions between astrocytes and neurons. The sensors are usually well characterized in vitro...
Tatsuma Yao et al.
Reproductive medicine and biology, 16(2), 99-117 (2017-12-21)
Cell culture technology has spread prolifically within a century, a variety of culture media has been designed. This review goes through the history, characteristics and current issues of animal-cell culture media. A literature search was performed on PubMed and Google...
Shafagh A Waters et al.
The EMBO journal, 36(3), 374-387 (2016-11-12)
RNA sequencing studies have identified hundreds of non-coding RNAs in bacteria, including regulatory small RNA (sRNA). However, our understanding of sRNA function has lagged behind their identification due to a lack of tools for the high-throughput analysis of RNA-RNA interactions...
Samuel Wilson Failor et al.
Neural development, 13(1), 4-4 (2018-03-27)
Activity in neurons drives afferent competition that is critical for the refinement of nascent neural circuits. In ferrets, when an eye is lost in early development, surviving retinogeniculate afferents from the spared eye spread across the thalamus in a manner...


MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service