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MilliporeSigma

M7279

N,N′-Methylenebisacrylamide

powder, Molecular Biology, suitable for electrophoresis, ≥99.5%

Synonym(s):

BIS

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About This Item

Linear Formula:
(CH2=CHCONH)2CH2
CAS Number:
110-26-9
Molecular Weight:
154.17
UNSPSC Code:
41105319
NACRES:
NB.22
PubChem Substance ID:
24897151
EC Number:
203-750-9
Beilstein/REAXYS Number:
1706297
MDL number:
MFCD00008625

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InChI key

ZIUHHBKFKCYYJD-UHFFFAOYSA-N

InChI

1S/C7H10N2O2/c1-3-6(10)8-5-9-7(11)4-2/h3-4H,1-2,5H2,(H,8,10)(H,9,11)

SMILES string

C=CC(=O)NCNC(=O)C=C

grade

Molecular Biology

assay

≥99.5%

form

powder

technique(s)

electrophoresis: suitable

impurities

≤0.05% Free carboxylic acid (T), ≤50 mg/kg Total sulfur (as SO4 ) (ICP)

conductivity

≤10 μmho (2% solution)

anion traces

chloride (Cl-): ≤50 mg/kg

cation traces

Ca: ≤10 ppm, Cd: ≤5 ppm, Co: ≤5 ppm, Cr: ≤5 ppm, Cu: ≤5 ppm, Fe: ≤5 ppm, K: ≤50 ppm, Mg: ≤5 ppm, Mn: ≤5 ppm, Na: ≤50 ppm, Ni: ≤5 ppm, Pb: ≤5 ppm, Zn: ≤5 ppm

absorption

≤0.2 at 290 at 1%

foreign activity

DNase and RNase, none detected

Quality Level

200

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1 of 4

This Item
M7256M1533146072
N,N′-Methylenebisacrylamide powder, Molecular Biology, suitable for electrophoresis, ≥99.5%

Sigma-Aldrich

M7279

N,N′-Methylenebisacrylamide

N,N′-Methylenebisacrylamide suitable for electrophoresis (after filtration or allowing insolubles to settle)

Sigma-Aldrich

M7256

N,N′-Methylenebisacrylamide

N,N′-Methylenebisacrylamide solution suitable for electrophoresis, 2% in H2O

Sigma-Aldrich

M1533

N,N′-Methylenebisacrylamide solution

N,N′-Methylenebis(acrylamide) 99%

Sigma-Aldrich

146072

N,N′-Methylenebis(acrylamide)

form

powder

form

powder

form

liquid

form

powder

grade

for molecular biology

grade

-

grade

-

grade

-

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

-

impurities

≤0.05% Free carboxylic acid (T), ≤50 mg/kg Total sulfur (as SO4 ) (ICP)

impurities

-

impurities

-

impurities

-

foreign activity

DNase and RNase, none detected

foreign activity

-

foreign activity

-

foreign activity

-

assay

≥99.5%

assay

-

assay

-

assay

99%

General description

N,N′-Methylenebisacrylamide is commonly called as bis. It is used as a cross-linking agent in the preparation of polyacrylamide gels consisting of chains of acrylamide monomers. The concentration of N,N′-methylenebisacrylamide and acrylamide determines the pore size of the gel. The higher concentration of bis forms rigid gels with smaller pores due to more cross-linking leading to polymerization and vice versa.[1]

Application

N,N′-Methylenebisacrylamide has been used:
  • as a crosslinker in the monomer solution to prepare N,N-dimethyl acrylamide (DMAAm)-based cryogel and acrylamide (AAm)-based cryogel matrix[2]
  • as a crosslinker to prepare tough hydrogel network[3]
  • in the preparation of acrylamide-bisacrylamide stock solution for the preparation of polyacrylamide gel[4]

To be mixed with acrylamide for making polyacrylamide gels for use in protein and nucleic acid electrophoresis.

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Carc. 1B - Muta. 1B - Repr. 2 - STOT RE 1 Oral

target_organs

Peripheral nervous system

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000507360
0000507360
0000465207
0000465207
0000481215

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Fundamentals and Techniques of Biophysics and Molecular Biology (2018)
Depletion of hemoglobin and carbonic anhydrase from erythrocyte cytosolic samples by preparative clear native electrophoresis
Damici GM, et al.
Nature Protocols, 7(1), 36-36 (2012)
Ultrastretchable Conductor Fabricated on Skin-Like Hydrogel-Elastomer Hybrid Substrates for Skin Electronics
Kim SH, et al.
Advanced Materials, 30(26), 1800109-1800109 (2018)
Ashok Kumar et al.
Nature protocols, 5(11), 1737-1747 (2010-10-30)
In cell affinity chromatography, type-specific cell separation is based on the interaction between cell-surface receptors and an immobilized ligand on a stationary matrix. This protocol describes the preparation of monolithic polyacrylamide and polydimethylacrylamide cryogel affinity matrices that can be used
Ghazaleh Haghiashtiani et al.
Science advances, 6(35), eabb4641-eabb4641 (2020-09-15)
Minimally invasive surgeries have numerous advantages, yet complications may arise from limited knowledge about the anatomical site targeted for the delivery of therapy. Transcatheter aortic valve replacement (TAVR) is a minimally invasive procedure for treating aortic stenosis. Here, we demonstrate
View All Related Papers

Questions

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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