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M7403

Sigma-Aldrich

MCDB 153 Medium

With trace elements, L-glutamine and 28mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

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Synonym(s):
Molecular, Cellular, and Development Biology 153 medium
NACRES:
NA.75

Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
L-glutamine: 0.8772 g/L
glucose: 1.081 g/L (Dextro)
sodium pyruvate: 0.055 g/L
phenol red: 0.001242 g/L
HEPES: 6.6 g/L (25mM)

shipped in

ambient

storage temp.

2-8°C

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M6770M3769M2520
vibrant-m

M7403

MCDB 153 Medium

vibrant-m

M6770

MCDB 201 Medium

vibrant-m

M3769

Medium 199

vibrant-m

M2520

Medium 199

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
L-glutamine: 0.8772 g/L
glucose: 1.081 g/L (Dextro)
sodium pyruvate: 0.055 g/L
phenol red: 0.001242 g/L
HEPES: 6.6 g/L (25mM)

components

HEPES: 7.149 g/L (30mM)
glucose: 1.441 g/L (Dextro)
phenol red: 0.001242 g/L
sodium pyruvate: 0.055 g/L
L-glutamine: 0.14615 g/L

components

L-glutamine: no
phenol red: no
HEPES: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: no

components

L-glutamine: yes
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
sodium pyruvate: no
HEPES: 25 mM

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

500

Quality Level

500

Quality Level

500

Quality Level

500

General description

MCDB 153 media are modifications of Ham′s nutrient mixture F-12, designed for the growth of human keratinocytes, clonal growth of chicken embryo fibroblasts, and Chinese hamster ovary (CHO) cells using low levels of fetal bovine serum protein (FBSP), extensive trace elements or no serum protein.

Application

MCDB 153 Medium has been used as a component of melanoma isolation media (MIM) to culture human non-metastatic and metastatic melanoma cells.

Quantity

Formulated to contain 17.6 grams of powder per liter of medium.

Reconstitution

Supplement with 1.18 g/L sodium bicarbonate.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Protocols

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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