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M7523

Sigma-Aldrich

Anti-Myosin (Skeletal) antibody produced in rabbit

enhanced validation

whole antiserum

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Synonym(s):
Anti-CPSFS1A, Anti-CPSFS1B, Anti-CPSKF1A, Anti-CPSKF1B, Anti-DA2A, Anti-DA2B, Anti-DA2B3, Anti-DA8, Anti-HEMHC, Anti-MYHC-EMB, Anti-MYHSE1, Anti-SMHCE
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

species reactivity

human

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using human or animal skeletal muscle
indirect immunofluorescence: 1:20 using human or animal sletetal muscle

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

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M7648M1570M4276
Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

antibody form

whole antiserum

antibody form

whole antiserum

antibody form

purified immunoglobulin

antibody form

ascites fluid

biological source

rabbit

biological source

rabbit

biological source

mouse

biological source

mouse

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

species reactivity

human

species reactivity

animal, human

species reactivity

rat, chicken, rabbit, mouse, human, bovine, guinea pig, feline

species reactivity

rat, chicken, rabbit, mouse, human, bovine, guinea pig, feline

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General description

Myosin is a 480,000 dalton protein containing two identical heavy chains (200,000 daltons each) and four light chains (15,000-26,000 daltons). Myosin molecules consist of two major regions: tails (rods) and heads; they aggregate into filaments through the tail region. Multiple forms of myosin heavy chains exist for each muscle type; skeletal, cardiac, smooth and non-muscle isomyosin forms exist in different types of skeletal muscle, depending on the physiological function of the muscle.

Specificity

Specifically labels the A bands of human and animal skeletal muscle. Does not stain human smooth muscle tissue.

Immunogen

whole myosin (heavy and light chains) from human skeletal muscle.

Application

Anti-Myosin (Skeletal) antibody produced in rabbit has been used in:
  • immunohistochemistry
  • immunostaining
  • western blotting
  • immunofluorescence
  • immunolocalization
  • immunocytochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Western Blotting (1 paper)

Quality

The antiserum has been treated to remove lipoproteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Multipotent Stem Cells from Umbilical Cord: Cord Is Richer than Blood!
Secco M, et al.
Stem Cells, 26(1), 146-150 (2008)
Tatiana Jazedje et al.
Journal of translational medicine, 7, 46-46 (2009-06-23)
The possibility of using stem cells for regenerative medicine has opened a new field of investigation. The search for sources to obtain multipotent stem cells from discarded tissues or through non-invasive procedures is of great interest. It has been shown
Human fallopian tube: a new source of multipotent adult mesenchymal stem cells discarded in surgical procedures
Jazedje T, et al.
Journal of Translational Medicine, 7(1), 1-10 (2009)
Myosin: The Actin Motor Protein
Molecular Cell Biology. 4th edition. (2000)
Małgorzata Zimowska et al.
International journal of molecular sciences, 21(9) (2020-05-13)
Skeletal muscle regeneration depends on the satellite cells, which, in response to injury, activate, proliferate, and reconstruct damaged tissue. However, under certain conditions, such as large injuries or myopathies, these cells might not sufficiently support repair. Thus, other cell populations

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