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M7769

Sigma-Aldrich

IgG2a, Kappa from murine myeloma

clone UPC 10, ascites fluid, lyophilized powder

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Synonym(s):
Mouse IgG2a-κ
MDL number:
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

clone

UPC 10, monoclonal

form

lyophilized powder

storage temp.

2-8°C

target post-translational modification

unmodified

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General description

The UPC-10 tumor line that produces mouse IgG2a, K is a tetramethylpentadecane (pristane) induced plasmacytoma originated and carried intraperitoneally in BALB/c mice. The hapten binding specificity of the UPC-10 line is for β-2-6-linked fructosan. The ascites produced from the UPC-10 tumor line contains IgG2a, K in addition to normal levels of other mouse immunoglobulins and serum proteins. The ascites fluid is clarified by centrifugation and filtration. IgG2a (immunoglobulin G 2a) is one of the subclasses of rat immunoglobulins, which also include IgG1, IgG2b and IgG2c.

Specificity

Antigen Specificity: β-2,6-fructosan

Trace amounts of lambda chain may be present.

Immunogen

The UPC-10 tumor line that produces mouse IgG2a, K is a tetramethylpentadecane (pristane) induced plasmacytoma originated and carried intraperitoneally in BALB/c mice.

Application

IgG2a, Kappa from murine myeloma has been used in immunofluorescence and immunohistochemistry.

Biochem/physiol Actions

The IgG2a (immunoglobulin G 2a) plays a vital role in stimulating autoimmunity in mice. This gene is expressed at high level during virus infection. IgG2a offers host defence through activation of complement system. This antibody has specificity towards Fc receptors expressed on phagocytes and stimulates antibody-dependent cell mediated cytotoxicity. IgG2a also induces macrophage-mediated opsonophagocytosis, which has been observed in response to influenza virus and leads to its clearance from the infected cells.

Physical form

The product provided is lyophilized from 0.01 M phosphate buffered saline, pH 7.2, with no preservatives added.

Preparation Note

Storage and Stability: Store the product at 2-8 °C. After reconstitution, the solution may be stored frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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