All Photos(1)

M7939

Sigma-Aldrich

Anti-Myosin IIB, non-muscle antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~200 kDa

species reactivity

rat, canine, human

technique(s)

western blot (chemiluminescent): 1:200 using whole cell extracts of cultured dog MDCK kidney cells, cultured rat NRK cells, and cultured human Jurkat cells.

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

General description

Myosin IIB (nonmuscle) is a relatively abundant, widespread two headed myosin composed of an N-terminal motor domain, a light chain binding neck region, a coiled-coil region, and a nonhelical C-terminal domain. It forms a heterohexamer composed of a pair of heavy chains and two pairs of light chains. Myosin IIB is expressed in embryonic cardiac myocytes.

Specificity

Anti-Myosin IIB, non-muscle antibody is specific for the heavy chain of human myosin IIB (nonmuscle). The antibody also reacts with myosin in dogs and rats.

Immunogen

Synthetic peptide corresponding to amino acid residues 1965-1976 of the heavy chain of human myosin IIB (nonmuscle) conjugated to KLH. This sequence is identical in rabbit and differs by one and two amino acids from the respective bovine and rat sequences.

Application

Anti-Myosin IIB, non-muscle antibody produced in rabbit has been used in:
  • immunoblotting
  • stimulated emission depletion (STED) nanoscopy
  • immunofluorescence

Biochem/physiol Actions

Myosin IIB plays a key role in the COS-7 cell cytokinesis and is need for the initiation of ring constriction.
Myosins are actin-dependent motors that have 15 classes based on their phylogenetics . Myosin IIb regulates memory and synaptic plasticity functions. Nonmuscle myosin IIb has been implicated in fibroblast movement and erythroblast enucleation .

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Myosin II recruitment during cytokinesis independent of centralspindlin-mediated phosphorylation
Beach JR and Egelhoff TT
Test, 284(40), 27377-27383 (2009)
Non-muscle myosin heavy chain IIA and IIB interact and co-localize in living cells: relevance for MYH9-related disease
Marini M, et al.
International Journal of Molecular Medicine, 17(5), 729-736 (2006)
The role of pulmonary vascular contractile protein expression in pulmonary arterial hypertension
Konik EA, et al.
Journal of Molecular and Cellular Cardiology, 65(12), 147-155 (2013)
Life without double-headed non-muscle myosin II motor proteins
Betapudi V
Frontiers in Chemistry, 2, 45-45 (2014)
Localization of sarcomeric proteins during myofibril assembly in cultured mouse primary skeletal myotubes
White J, et al.
The Anatomical Record, 297(9), 1571-1584 (2014)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service