M8064

Sigma-Aldrich

Anti-Myosin IIA, non muscle antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~200 kDa

species reactivity

human, rat, canine

packaging

antibody small pack of 25 μL

application(s)

indirect immunofluorescence: 1:100 using cultured rat NRK cells
microarray: suitable
western blot: 1:1,000 using whole cell extracts of cultured dog MDCK kidney cells and cultured human Jurkat cells

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... MYH9(4627)
rat ... Myh9(25745)

Related Categories

General description

Myosin heavy chain 9 (MYH9) gene codes for the nonmuscle myosin heavy chain IIA.
Myosin heavy chain 9 is expressed in platelets and the gene encoding it is localized on chromosome 22.

Immunogen

synthetic peptide corresponding to amino acids 1949-1960 of human nonmuscle myosin IIA.

Application

Anti-Myosin IIA, non muscle antibody produced in rabbit has been used in:
  • immunofluorescence
  • immunohistochemistry
  • fixed cell staining/ immunofluorescence staining
  • immunoblot
  • immunoprecipitation

Biochem/physiol Actions

Nonmuscle myosin II is involved in cell motility and adhesion, cytokinesis, vesicular transport, intracellular force generation and in morphogenesis during development. Its activity is regulated by light chain and possibly heavy chain phosphorylation and by association with proteins such as Mts1. Mutations in the NMHCA gene are found in several syndromes associated with megakaryocyte/platelet/leukocyte disorders. Mutations in the MYH9 gene causes a spectrum of macrothrombocytopenia disorders with neutrophil inclusions, termed as MYH9 disorders.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Adhesion-dependent and contractile ring-independent equatorial furrowing during cytokinesis in mammalian cells
Kanada M, et al.
Molecular Biology of the Cell, 16(8), 3865-3872 (2005)
The role of pulmonary vascular contractile protein expression in pulmonary arterial hypertension
Konik EA, et al.
Journal of Molecular and Cellular Cardiology, 65, 147-155 (2013)
The role of stress fibers in the shape determination mechanism of fish keratocytes
Nakata T, et al.
Biophysical Journal, 110(2), 481-492 (2016)
Nonmuscle myosin-2: mix and match
Heissler SM and Manstein DJ
Cellular and Molecular Life Sciences, 70(1), 1-21 (2013)
Y Chen et al.
Journal of thrombosis and haemostasis : JTH, 11(12), 2163-2175 (2013-10-30)
Mutations in the MYH9 gene cause autosomal dominant MYH9-related diseases (MYH9-RD) that associate macrothrombocytopenia with various other clinical conditions. The mechanisms giving rise to giant platelets remain poorly understood. To study the proplatelet formation (PPF) derived from megakaryocytes (MKs) generated...

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