M8069

Monoclonal Anti-Mad1 antibody produced in mouse

~2 mg/mL, clone 9B10, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-Mitotic Arrest Deficient
• MDL number: MFCD08705588
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 9B10, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~85 kDa
• species reactivity: human
• concentration: ~2 mg/mL
• technique(s): immunocytochemistry: 10-20 μg/mL using HeLa cells; immunoprecipitation (IP): suitable; microarray: suitable
• isotype: IgG2b
• UniProt accession no.: Q9Y6D9
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MAD1L1(8379)

Description

General description

Mitotic arrest deficient-like 1 (MAD1) is part of the cell-cycle checkpoint.

Monoclonal Anti-Mad1 (mouse IgG2b isotype) is derived from the hybridoma 9B10 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with human recombinant Mad1 protein.

Immunogen

recombinant human Mad1 protein.

Application

Monoclonal Anti-Mad1 antibody produced in mouse has been used in

• immunoblotting
• immunoprecipitation
• immunocytochemistry

Biochem/physiol Actions

Mitotic arrest deficient-like 1 (MAD1) functions as a regulator in the segregation of chromosomes during mitosis. It recruits mitotic arrest deficient-like 2 (MAD2) to kinetochores and directs it to function as an inhibitor. MAD1 also helps MAD2 to associate with translocated promoter region (TPr).

Physical form

Solution in 0.01 M phosphae buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable