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M8069

Sigma-Aldrich

Monoclonal Anti-Mad1 antibody produced in mouse

~2 mg/mL, clone 9B10, purified immunoglobulin, buffered aqueous solution

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Synonym(s):
Anti-Mitotic Arrest Deficient
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9B10, monoclonal

form

buffered aqueous solution

mol wt

antigen ~85 kDa

species reactivity

human

concentration

~2 mg/mL

technique(s)

immunocytochemistry: 10-20 μg/mL using HeLa cells
immunoprecipitation (IP): suitable
microarray: suitable

isotype

IgG2b

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MAD1L1(8379)

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MABE867SAB4503302C7104
vibrant-m

MABE867

Anti-Mad1 Antibody, clone BB3-8

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified from hybridoma cell culture

Gene Information

human ... MAD1L1(8379)

Gene Information

human ... MAD1L1(8379)

Gene Information

human ... MAD1L1(8379)

Gene Information

human ... CDC27(996)
mouse ... Cdc27(217232)
rat ... Cdc27(360643)

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

mouse, Xenopus, rat, bovine, canine, human

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

mouse

clone

9B10, monoclonal

clone

BB3-8, monoclonal

clone

polyclonal

clone

AF3.1, monoclonal

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General description

Mitotic arrest deficient-like 1 (MAD1) is part of the cell-cycle checkpoint.
Monoclonal Anti-Mad1 (mouse IgG2b isotype) is derived from the hybridoma 9B10 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with human recombinant Mad1 protein.

Immunogen

recombinant human Mad1 protein.

Application

Monoclonal Anti-Mad1 antibody produced in mouse has been used in
  • immunoblotting
  • immunoprecipitation
  • immunocytochemistry

Biochem/physiol Actions

Mitotic arrest deficient-like 1 (MAD1) functions as a regulator in the segregation of chromosomes during mitosis. It recruits mitotic arrest deficient-like 2 (MAD2) to kinetochores and directs it to function as an inhibitor. MAD1 also helps MAD2 to associate with translocated promoter region (TPr).

Physical form

Solution in 0.01 M phosphae buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Jun Wan et al.
Current biology : CB, 24(22), 2687-2692 (2014-12-03)
Mitotic arrest deficient 1 (Mad1) plays a well-characterized role in the major cell-cycle checkpoint that regulates chromosome segregation during mitosis, the mitotic checkpoint (also known as the spindle assembly checkpoint). During mitosis, Mad1 recruits Mad2 to unattached kinetochores, where Mad2
Timo E F Kuijt et al.
Chromosoma, 123(5), 471-480 (2014-04-04)
Fidelity of chromosome segregation is monitored by the spindle assembly checkpoint (SAC). Key components of the SAC include MAD1, MAD2, BUB1, BUB3, BUBR1, and MPS1. These proteins accumulate on kinetochores in early prometaphase but are displaced when chromosomes attach to
Garry G Sedgwick et al.
mAbs, 8(4), 689-697 (2016-03-18)
The spindle assembly checkpoint (SAC) ensures accurate chromosome segregation during mitosis by delaying the activation of the anaphase-promoting complex/cyclosome (APC/C) in response to unattached kinetochores. The Mad2 protein is essential for a functional checkpoint because it binds directly to Cdc20
Gang Zhang et al.
The EMBO journal, 38(7) (2019-02-21)
Kinetochore localized Mad1 is essential for generating a "wait anaphase" signal during mitosis, hereby ensuring accurate chromosome segregation. Inconsistent models for the function and quantitative contribution of the two mammalian Mad1 kinetochore receptors: Bub1 and the Rod-Zw10-Zwilch (RZZ) complex exist.
Arindam Chakraborty et al.
Nature communications, 5, 3445-3445 (2014-03-13)
Heterochromatin protein 1α (HP1α), a key player in the establishment and maintenance of higher-order chromatin regulates key cellular processes, including metaphase chromatid cohesion and centromere organization. However, how HP1α controls these processes is not well understood. Here we demonstrate that

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