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M8250

Sigma-Aldrich

MES hydrate

≥99.5% (titration)

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Synonym(s):
2-(N-Morpholino)ethanesulfonic acid hydrate, 4-Morpholineethanesulfonic acid
Empirical Formula (Hill Notation):
C6H13NO4S · xH2O
CAS Number:
Molecular Weight:
195.24 (anhydrous basis)
MDL number:
eCl@ss:
32129211
PubChem Substance ID:

Quality Level

assay

≥99.5% (titration)

form

crystalline powder

useful pH range

5.5-6.7

pKa 

6.1

solubility

water: 0.25 g/mL, clear, colorless

application(s)

agriculture
clinical research
diagnostic assay manufacturing
life science and biopharma

storage temp.

room temp

SMILES string

O.OS(=O)(=O)CCN1CCOCC1

InChI

1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2

InChI key

MIIIXQJBDGSIKL-UHFFFAOYSA-N

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1 of 4

This Item
M293369890PHG0003
vibrant-m

M8250

MES hydrate

-
vibrant-m

M2933

MES hydrate

Premium Grade
vibrant-m

69890

MES hydrate

Premium Grade
vibrant-m

PHG0003

MES hydrate

-
Quality Level

400

Quality Level

400

Quality Level

200

Quality Level

400

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

form

crystalline powder

form

crystalline powder

form

powder or crystals

form

powder

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

-

solubility

water: 0.25 g/mL, clear, colorless

solubility

-

solubility

H2O: 0.5 M at 20 °C, clear, colorless

solubility

-

General description

MES hydrate, or 4-morpholineethanesulfonic acid hydrate, is a zwitterionic and morpholinic buffer renowned as one of the "Good′s" buffer for biological and biochemical applications. Its outstanding buffering capacity in the pH range of 5.5 to 6.7 makes it versatile for a wide array of research applications. Notably, MES Buffer minimizes interaction with common metals used in environmental and biological studies, preventing interference with experimental outcomes.

With excellent water solubility and virtual insolubility in lipids, MES Buffer is impermeable to membranes, ensuring efficacy in cellular environments. Its applications span maintaining stability in cell culture media, protein-based buffer formulations, and electrophoresis running buffers. MES Buffer may also find usage in purifying antibodies, peptides, proteins, blood components, and growth factors. MES Buffer stands out as a better alternative to potentially toxic agents like cacodylate and non-zwitterionic buffers such as citrate and malate. MES Buffer proves valuable for adjusting growth media pH, stabilizing enzymatic solutions, and serving as a component of PAGE running buffer in diverse experimental settings.

Application

MES hydrate has been used:
  • in the MS (Murashige and Skoog) media for growth of Arabidopsis seedlings.
  • in the cytoskeleton buffer during immunofluorescence study.
  • as a wash buffer to wash the paramagnetic beads and resuspend them for binding mannose-BSA
  • as a component of growth medium

Features and Benefits

  • Effective Buffering from pH 5.5-6.7 with a pKa of 6.1 (25 °C)
  • Highly soluble in water
  • Minimal metal ion binding
  • Stable in a wide pH range
  • Low UV absorptivity and Minimal reactivity

Preparation Note

A buffer using MES can be prepared by titrating with NaOH to the desired pH. Alternatively, stock solutions of MES and MES sodium salt can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare buffers of a given pH have been published. MES is not recommended for buffering at pH 7.4; other buffers should be considered.

Storage and Stability

Solutions are stable at 2-8°C for months. Sterilize by filtration through 0.2uM filters. Autoclaving is not recommended for any sulfonic acid buffer. If buffers must be nuclease-free, treat the water first, and then add the buffer after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


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-
25G
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705578-5MG-PW

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MMYOMAG-74K-13

1000309185

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Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

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  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

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Proteomic and phosphoproteomic analyses of chromatin-associated proteins from Arabidopsis thaliana.
Bigeard J et al.
Proteomics, 14, 2141-2141 (2014)
Data for Biochemical Research, Dawson, R.M.C. et al, 410-410 (1987)
"(Un) suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions-a review.
Ferreira, Carlos MH, et al.
Royal Society of Chemistry Advances, 5 (39), 30989-31003 (2015)
Negative effect of heat shock on feline calicivirus release from infected cells is associated with the control of apoptosis.
Alvarez-Sanchez C et al.
Virus Research, 198, 44-44 (2015)
Good, Norman E. et al.
Biochemistry, 5, 467- 477 (1966)

Protocols

This procedure may be used for Mutanolysin products.

Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)

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