MilliporeSigma
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M8642

Sigma-Aldrich

Anti-Mouse IgG (whole molecule) antibody produced in goat

affinity isolated antibody, lyophilized powder

MDL number:

biological source

goat

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

lyophilized powder

technique(s)

Ouchterlony double diffusion: suitable

storage temp.

2-8°C

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This Item
M8890M5899M8645
biological source

goat

biological source

goat

biological source

goat

biological source

goat

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

antibody form

whole antiserum

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

form

lyophilized powder

form

lyophilized powder

form

-

form

lyophilized powder

technique(s)

Ouchterlony double diffusion: suitable

technique(s)

Ouchterlony double diffusion: 1:8

technique(s)

indirect ELISA: 1:70,000, quantitative precipitin assay: 3.2-4.8 mg/mL

technique(s)

Ouchterlony double diffusion: suitable

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Anti-Mouse IgG (whole molecule) antibody recognizes IgG1, G2a, G2b, and G3, mouse IgM and IgA. The identity and purity of affinity isolated antibody is established by immunoelectrophoresis.

Immunogen

Pooled normal mouse serum

Application

Anti-Mouse IgG (whole molecule) antibody was used as secondary antibody in immunoblotting at a working dilution of 1:1000. The antibody was used in ELISA and RIA in various studies.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)

Physical form

Lyophilized from 0.01 M sodium phosphate, 0.015 M sodium chloride, pH 7.2

Reconstitution

Reconstitute with 0.135 M sodium chloride.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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