M8662

Mineral oil

PCR Reagent

• Synonym(s): Mineral oil for PCR
• CAS Number: 8042-47-5
• UNSPSC Code: 12181504
• NACRES: NA.52
• EC Number: 232-455-8
• MDL number: MFCD00131611
• eCl@ss: 26010105
• Technique(s): PCR: suitable, whole genome amplification: suitable

Properties

• Quality Segment: 100
• form: liquid
• feature: PCR Additives
• packaging: vial of 1.5 mL (Total volume 7.5 mL (5 vials))
• technique(s): PCR: suitable, whole genome amplification: suitable
• color: colorless
• refractive index: n20/D 1.467 (lit.)
• density: 0.84 g/mL at 25 °C (lit.)
• application(s): cannabis testing
• foreign activity: DNase, RNase, protease, none detected
• storage temp.: room temp
• InChI key: AEOVEGJBKQQFOP-DDVLFWKVSA-L

Description

General description

Mineral oil is used as a reaction mix overlay in polymerase chain reactions (PCR) to prevent reaction solutions from evaporating during the thermal cycling in PCR instruments without heated lids.

Application

Mineral oil has been used:

• for routine PCR amplifications
• to improve the heat conductivity between the device and the cycler during reverse transcription-polymerase chain reaction (RT–PCR) amplification
• to prevent evaporation during single-cell MATQ-sequencing performed on Bravo automated liquid handling platform

Features and Benefits

• Provided in a convenient 5 × 1.5 mL (1 vial) pack size
• Tested for the absence of DNase, RNase, and protease.
  

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): No data available
• Flash Point (°C): No data available
• PPE (personal protective equipment): Eyeshields, Gloves