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M8662

Sigma-Aldrich

Mineral oil

PCR Reagent

Synonym(s):

Mineral oil for PCR

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5 VIALS
$108.00

$108.00

Available to ship onMay 13, 2025Details

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5 VIALS
$108.00

About This Item

CAS Number:
8042-47-5
EC Number:
232-455-8
MDL number:
MFCD00131611
UNSPSC Code:
12181504
NACRES:
NA.52
technique(s):
PCR: suitable

$108.00

Available to ship onMay 13, 2025Details

Quality Level

100

form

liquid

packaging

vial of 1.5 mL (Total volume 7.5 mL (5 vials))

technique(s)

PCR: suitable

color

colorless

refractive index

n20/D 1.467 (lit.)

density

0.84 g/mL at 25 °C (lit.)

foreign activity

DNase, RNase, protease, none detected

storage temp.

room temp

InChI key

AEOVEGJBKQQFOP-DDVLFWKVSA-L

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General description

Mineral oil is used as a reaction mix overlay in polymerase chain reactions (PCR) to prevent reaction solutions from evaporating during the thermal cycling in PCR instruments without heated lids.[1]

Application

Mineral oil has been used:
  • for routine PCR amplifications
  • to improve the heat conductivity between the device and the cycler during reverse transcription-polymerase chain reaction (RT–PCR) amplification[2]
  • to prevent evaporation during single-cell MATQ-sequencing performed on Bravo automated liquid handling platform[3][4]

Features and Benefits

  • Provided in a convenient 5 × 1.5 mL (1 vial) pack size
  • Tested for the absence of DNase, RNase, and protease.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

No data available

flash_point_c

No data available

ppe

Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
MKCW2105
MKCN0398
MKCK1218
MKCF5776
MKCC0168

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Hsin-I Jen et al.
eLife, 8 (2019-04-30)
The mammalian cochlea loses its ability to regenerate new hair cells prior to the onset of hearing. In contrast, the adult vestibular system can produce new hair cells in response to damage, or by reprogramming of supporting cells with the
Hartmut Kaiser et al.
Plant physiology, 143(2), 1068-1077 (2006-12-13)
The response of stomata to a reduction of air humidity is composed of a hydropassive opening followed by active closure. Whereas the mechanisms behind the hydropassive opening are largely understood, the location and physiological basis of the sensing mechanisms leading
Ivan K Dimov et al.
Nature communications, 5, 3451-3451 (2014-03-29)
Discriminating cellular heterogeneity is important for understanding cellular physiology. However, it is limited by the technical difficulties of single-cell measurements. Here we develop a two-stage system to determine cellular heterogeneity. In the first stage, we perform multiplex single-cell RNA cytometry
Decagram scale production of deuterated mineral oil and polydecene as solvents for polymer studies in neutron scattering
Klenner MA, et al.
Open Journal of Polymer Chemistry, 11(31), 4986-4994 (2020)
Neus Bota-Rabassedas et al.
Cell reports, 35(3), 109009-109009 (2021-04-22)
Cancer cells function as primary architects of the tumor microenvironment. However, the molecular features of cancer cells that govern stromal cell phenotypes remain unclear. Here, we show that cancer-associated fibroblast (CAF) heterogeneity is driven by lung adenocarcinoma (LUAD) cells at
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Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Long & Accurate PCR with RedAccuTaq®

REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.

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