Mitotic arrest deficient 2 (MAD2) can exist in different folded states and is one of the components of the spindle checkpoint.
Monoclonal Anti-Mad2 (mouse IgG1 isotype) is derived from the hybridoma 17D10 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice. MAD1 gene is mapped to human chromosome 4q27. It exists in monomeric and tetrameric forms.
Monoclonal Anti-Mad2 recognizes human Mad2.
recombinant human Mad2 protein.
Anti-Mad2 antibody, Mouse monoclonal has been used in:
- western blotting
- immunofluorescence microscopy
Mitotic arrest deficient 2 (MAD2) plays a very important role in maintaining the number of chromosomes at the time of cell division. It checks the loss or gain of chromosomes. It delays the separation of premature sister-chromatids by acting on different proteins.It is overexpressed in many types of cancers.
MAD1 can complex with MAD2 and preferentially localize to unattached kinetochores during mitosis. During interphase, MAD1 and MAD2 localizes to the nuclear pore complex.
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
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