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M8694

Sigma-Aldrich

Anti-Mad2 antibody,Mouse monoclonal

clone 17D10, purified from hybridoma cell culture

Synonym(s):
Anti-MAD2L1, Anti-Mitotic Arrest Deficient 2
MDL number:

Quality Level

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

17D10, monoclonal

form

buffered aqueous solution

mol wt

antigen ~25 kDa

species reactivity

human

concentration

~2 mg/mL

application(s)

immunoprecipitation (IP): suitable
microarray: suitable
western blot: 2-4 μg/mL using total cell extract of HeLa cells

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... MAD2L1(4085)

General description

Mitotic arrest deficient 2 (MAD2) can exist in different folded states and is one of the components of the spindle checkpoint.
Monoclonal Anti-Mad2 (mouse IgG1 isotype) is derived from the hybridoma 17D10 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice. MAD1 gene is mapped to human chromosome 4q27. It exists in monomeric and tetrameric forms.

Specificity

Monoclonal Anti-Mad2 recognizes human Mad2.

Immunogen

recombinant human Mad2 protein.

Application

Anti-Mad2 antibody, Mouse monoclonal has been used in:
  • western blotting
  • immunohistochemistry
  • immunofluorescence microscopy
  • immunoprecipitation

Biochem/physiol Actions

Mitotic arrest deficient 2 (MAD2) plays a very important role in maintaining the number of chromosomes at the time of cell division. It checks the loss or gain of chromosomes. It delays the separation of premature sister-chromatids by acting on different proteins.It is overexpressed in many types of cancers.
MAD1 can complex with MAD2 and preferentially localize to unattached kinetochores during mitosis. During interphase, MAD1 and MAD2 localizes to the nuclear pore complex.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificate of Analysis

Certificate of Origin

Chaoqun Li et al.
International journal of molecular sciences, 15(4), 5553-5569 (2014-04-03)
The Mad2 protein, with two distinct conformations of open- and closed-states, is a key player in the spindle checkpoint. The closed Mad2 state is more active than the open one. We carried out conventional and targeted molecular dynamics simulations for...
M S Campbell et al.
Journal of cell science, 114(Pt 5), 953-963 (2001-02-22)
Mad1 was first identified in budding yeast as an essential component of the checkpoint system that monitors spindle assembly in mitosis and prevents premature anaphase onset. Using antibodies to the human homologue of Mad1 (HsMAD1), we have begun to characterize...
Mayuko Hara et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(36), 11252-11257 (2015-08-26)
The spindle checkpoint senses unattached kinetochores during prometaphase and inhibits the anaphase-promoting complex or cyclosome (APC/C), thus ensuring accurate chromosome segregation. The checkpoint protein mitotic arrest deficient 2 (Mad2) is an unusual protein with multiple folded states. Mad2 adopts the...
Lucia Sironi et al.
The EMBO journal, 21(10), 2496-2506 (2002-05-15)
The spindle checkpoint protein Mad1 recruits Mad2 to unattached kinetochores and is essential for Mad2-Cdc20 complex formation in vivo but not in vitro. The crystal structure of the Mad1-Mad2 complex reveals an asymmetric tetramer, with elongated Mad1 monomers parting from...
Yang Bian et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(4), 1628-1633 (2014-01-16)
The spindle checkpoint is essential to ensure proper chromosome segregation and thereby maintain genomic stability. Mitotic arrest deficiency 2 (Mad2), a critical component of the spindle checkpoint, is overexpressed in many cancer cells. Thus, we hypothesized that Mad2 overexpression could...

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