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M8697

Sigma-Aldrich

MEK1 (EE), active, GST tagged from mouse

PRECISIO® Kinase, recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

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Synonym(s):
MAPKK1, MKK1, PRKMK1
NACRES:
NA.32

recombinant

expressed in E. coli

Quality Level

product line

PRECISIO® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

276-374 nmol/min·mg

mol wt

~69 kDa

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

mouse ... Map2k1(26395)

General description

Mitogen-activated protein kinases 1 (MEK1) is encoded by the gene mapped to human chromosome 15q22.31 and the gene contains 11 exons with intervening sequences. MEK1 belongs to the MEK protein family and members of this family contain kinase domain at their amino-terminal and a catalytic domain at their carboxyl-terminal end.

Biochem/physiol Actions

Mitogen-activated protein kinase 1 (MEK1) participates in MAPK/ ERK (extracellular signal-regulated kinases) pathway, which plays a vital role in regulation of cell proliferation, differentiation and survival. Mutation of MEK1 gene leads to non-small cell lung cancer (NSCLC). In addition, aberrations of the gene is also observed in adenocarcinoma patients with current or former smoking status. MEK1 influences ERK activation in MAPK pathway and induce proliferation and anti-apoptotic signals. MEK1 facilitates platinum resistance via activation of excision repair cross-complementation group 1 (ERCC1) protein. Clinical trials show that, Trametinib acts as a MEK1/MEK2 inhibitor and is used to treat various cancers caused by MEK1 mutation including melanoma, colorectal cancer.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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MEK1 is associated with carboplatin resistance and is a prognostic biomarker in epithelial ovarian cancer.
Penzvalto Z
BMC Cancer, 14:837 (2014)
MEK and the inhibitors: from bench to bedside.
Akinleye A
Journal of Hematology & Oncology, 6:27 (2013)
Sensitive methods for screening of the MEK1 gene mutations in patients with central nervous system metastases of non-small cell lung cancer.
Nicos M
Clinical And Translational Oncology, 18(10), 1039-1043 (2016)
J S Sebolt-Leopold et al.
Nature medicine, 5(7), 810-816 (1999-07-08)
The mitogen-activated protein kinase pathway is thought to be essential in cellular growth and differentiation. Here we report the discovery of a highly potent and selective inhibitor of the upstream kinase MEK that is orally active. Tumor growth was inhibited
R Seger et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 9(9), 726-735 (1995-06-01)
The transmission of extracellular signals into their intracellular targets is mediated by a network of interacting proteins that regulate a large number of cellular processes. Cumulative efforts from many laboratories over the past decade have allowed the elucidation of one

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