M8823

Millipore

Anti-FLAG® M2 Magnetic Beads

affinity isolated antibody

Synonym(s):
Anti-ddddk, Anti-dykddddk, Flag Affinity resin, Monoclonal ANTI-FLAG M2 antibody produced in mouse, FLAG magnetic affinity resin, FLAG resin for high throughput
Pricing and availability is not currently available.

antibody form

affinity isolated antibody

Quality Level

antibody product type

primary antibodies

clone

M2, monoclonal

form

(Supplied as a 50% suspension in 50% glycerol with 10mM sodium phosphate, 150mM sodium chloride, pH 7.4 and 0.02% (w/v) sodium azide (PBA/A).)

shelf life

2 yr at -20 °C

analyte chemical class(es)

proteins (FLAG® Affinity Gels, FLAG® tag, 3x FLAG® tag, DYKDDDDK tag)

application(s)

affinity chromatography: suitable
immunoprecipitation (IP): suitable

bead size

20-75 μm

matrix

superparamagnetic iron impregnated 4% agarose bead, with an average diameter of 50 μm.

isotype

IgG1

capacity

≥0.6 mg/mL binding capacity

conjugate

magnetic beads

shipped in

wet ice

storage temp.

−20°C

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General description

Anti-FLAG M2 Magnetic Beads are 4% agarose beads bound with the Anti-FLAG M2 (mouse monoclonal) antibody. The M2 antibody recognizes the FLAG sequence at the N-terminus, Met-N-terminus and C-terminus. This alows for detection and capture of fusion proteins containing a FLAG peptide sequence.

Application

Suitable for immunoprecipitation procedures.

Elution - FLAG® peptide, Glycine, pH 3.5, 3x FLAG® peptide

Browse additional application references in our
FLAG® Literature portal.

Packaging

1 mL in poly bottle

Features and Benefits

The magnetic properties allow for:
- Very rapid separation
- Significantly accelerated manipulations, such as repetitive washings
- Processing of multiple samples performed in plate formats
This leads to:
- Faster experimentation
- Better reproducibility
- More accurate quantitation of the proteins of interest

Other Notes

Do not use magnetic stirring system as this will destroy the resin beads.

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Ephrem G Kassa et al.
PLoS pathogens, 15(6), e1007851-e1007851 (2019-06-27)
Enteropathogenic E. coli (EPEC) is an extracellular diarrheagenic human pathogen which infects the apical plasma membrane of the small intestinal enterocytes. EPEC utilizes a type III secretion system to translocate bacterial effector proteins into its epithelial hosts. This activity, which...
Chanqiong Zhang et al.
Cancer biology & therapy, 20(9), 1213-1222 (2019-04-16)
It is verified that long non-coding RNAs (lncRNAs) play crucial roles in various cancers. LncRNA LEF1-AS1 is a reported oncogene in colorectal cancer and glioblastoma. In this study, we unveiled that LEF1-AS1 markedly increased in oral squamous cell carcinoma (OSCC)...
K Kollmann et al.
Leukemia, 31(4), 934-944 (2016-10-16)
Most myeloproliferative neoplasm (MPN) patients lacking JAK2 mutations harbour somatic CALR mutations that are thought to activate cytokine signalling although the mechanism is unclear. To identify kinases important for survival of CALR-mutant cells, we developed a novel strategy (KISMET) that...
Long Yang et al.
Nature communications, 9(1), 2329-2329 (2018-06-15)
The ubiquitin regulatory X domain-containing proteins (UBXNs) are likely involved in diverse biological processes. Their physiological functions, however, remain largely unknown. Here we present physiological evidence that UBXN3B positively regulates stimulator-of-interferon genes (STING) signaling. We employ a tamoxifen-inducible Cre-LoxP approach...
Raghu R Edupuganti et al.
Nature structural & molecular biology, 24(10), 870-878 (2017-09-05)
RNA modifications are integral to the regulation of RNA metabolism. One abundant mRNA modification is N
Articles
The FLAG® Expression System is a proven method to express, purify and detect recombinant fusion proteins. Sigma®, the proven provider of FLAG®, now offers a magnetic bead for immunoprecipitation, protein purification, and the study of protein-protein interactions. The ANTI-FLAG® M2 Magnetic Bead is composed of murine derived, anti-FLAG® M2 monoclonal antibody attached to superparamagnetic iron impregated 4% agarose beads, with an average diameter of 50 µm. The M2 antibody is capable of binding to fusion proteins containing a FLAG peptide sequence at the N-terminus, Met-N-terminus, or C-terminus locations in mammalian, bacterial, and plant extracts.
Read More
Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.
Read More
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