M8823

Anti-FLAG^® M2 Magnetic Beads

affinity isolated antibody

• Synonym(s): Monoclonal ANTI-FLAG^® M2 antibody produced in mouse, FLAG^® magnetic affinity resin, FLAG^® resin for high throughput, Flag^® Affinity resin, Anti-ddddk, Anti-dykddddk

Properties

• conjugate: magnetic beads
• Quality Level: 200
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: M2, monoclonal
• form: suspension
• shelf life: 2 yr at -20 °C
• analyte chemical class(es): proteins (FLAG^® Affinity Gels, FLAG^® tag, 3x FLAG^® tag, DYKDDDDK tag)
• technique(s): affinity chromatography: suitable; immunoprecipitation (IP): suitable
• bead size: 20-75 μm
• matrix: superparamagnetic iron impregnated 4% agarose bead, with an average diameter of 50 μm.
• isotype: IgG1
• capacity: ≥0.6 mg/mL binding capacity
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Anti-FLAG M2 Magnetic Beads are 4% agarose beads bound with the Anti-FLAG M2 (mouse monoclonal) antibody. The M2 antibody recognizes the FLAG sequence at the N-terminus, Met-N-terminus and C-terminus. This alows for detection and capture of fusion proteins containing a FLAG peptide sequence.

Application

Suitable for immunoprecipitation procedures.

Elution - FLAG^® peptide, Glycine, pH 3.5, 3x FLAG^® peptide

Browse additional application references in our FLAG^® Literature portal.

Packaging

1, 5 mL

Features and Benefits

The magnetic properties allow for:
- Very rapid separation
- Significantly accelerated manipulations, such as repetitive washings
- Processing of multiple samples performed in plate formats
This leads to:
- Faster experimentation
- Better reproducibility
- More accurate quantitation of the proteins of interest

Physical form

Supplied as a 50% suspension in 50% glycerol with 10mM sodium phosphate, 150mM sodium chloride, pH 7.4 and 0.02% (w/v) sodium azide (PBA/A).

Other Notes

Do not use magnetic stirring system as this will destroy the resin beads.

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves