M9004

Malic Dehydrogenase from bovine heart

Name: Malic Dehydrogenase from bovine heart
Brand: SIGMA
Price: 281 USD

ammonium sulfate suspension, 2000-4000 units/mg protein (modified Warburg-Christian)

Synonym(s):

L-Malate:NAD⁺ oxidoreductase, MDH

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About This Item

EC Number:

1.1.1.37 (BRENDA, IUBMB)

EC Number:

232-622-5

MDL number:

MFCD00131567

UNSPSC Code:

12352204

NACRES:

NA.54

Key Documents

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biological source

bovine heart

Quality Level

200

form

ammonium sulfate suspension

specific activity

2000-4000 units/mg protein (modified Warburg-Christian)

mol wt

35 kDa

impurities

≤0.01% Glutamic-Oxalacetic Transaminase
≤0.01% Glutamic-Pyruvic Transaminase

storage temp.

2-8°C

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This Item	M1567	M2634	L2625
Sigma-Aldrich M9004 Malic Dehydrogenase from bovine heart	Sigma-Aldrich M1567 Malic Dehydrogenase from porcine heart	Sigma-Aldrich M2634 Malic Dehydrogenase from porcine heart	Sigma-Aldrich L2625 L-Lactic Dehydrogenase from bovine heart
specific activity 2000-4000 units/mg protein (modified Warburg-Christian)	specific activity ≥600 units/mg protein (biuret)	specific activity 600-1000 units/mg protein (biuret)	specific activity ≥500 units/mg protein
biological source bovine heart	biological source -	biological source -	biological source -
form ammonium sulfate suspension	form ammonium sulfate suspension	form buffered aqueous glycerol solution	form ammonium sulfate suspension
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
mol wt 35 kDa	mol wt -	mol wt -	mol wt -
impurities ≤0.01% Glutamic-Oxalacetic Transaminase, ≤0.01% Glutamic-Pyruvic Transaminase	impurities -	impurities -	impurities -

General description

Malic Dehydrogenase from bovine heart contains a histidine residue at the NAD-binding active site which is critical for activity. When this histidine is mutated a loss in activity is observed. [1]

Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle.

Application

Malic dehydrogenase has been used in a study to assess a flow injection system for on-line monitoring of fumaric acid in biological processes. [2] It has also been used in a study to investigate a root-knot nematode parasitizing peanut in Texas. [3]

Physical form

Suspension in 3 M (NH₄)₂SO₄ - 0.01 M KH₂PO₄ solution, pH 7.3

Other Notes

One unit will convert 1.0 μmole of oxalacetate and β-NADH to _L-malate and β-NAD per min at pH 7.5 at 25 °C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Meloidogyne haplanaria n. sp. (Nematoda: Meloidogynidae), a Root-knot Nematode Parasitizing Peanut in Texas.

J D Eisenback et al.

Journal of nematology, 35(4), 395-403 (2003-12-01)

Meloidogyne haplanaria n. sp. is described and illustrated from specimens parasitizing peanut in Texas. The perineal pattern of the female is rounded to oval with a dorsal arch that is high and rounded except for striae near the vulva, which

Isoelectric points and molecular weights of proteins: a new table.

Righetti, P.G., and Tudor, G.

Journal of Chromatography A, 220, 115-194 (1981)

A low-CO2-inducible gene encoding an alanine: alpha-ketoglutarate aminotransferase in Chlamydomonas reinhardtii.

Z Y Chen et al.

Plant physiology, 112(2), 677-684 (1996-10-01)

At low-CO2 (air) conditions, the unicellular green alga Chlamydomonas reinhardtii acquires the ability to raise its internal inorganic carbon concentration. To study this adaptation to low CO2, cDNA clones induced under low-CO2 growth conditions were selected through differential screening. One

Flow injection system for on-line monitoring of fumaric acid in biological processes

Rhee, J.

Analytica Chimica Acta, 499, 71-80 (2003)

Enzymatic assay of phosphatidylethanolamine in serum using amine oxidase from Arthrobacter sp.

Eisaku Hokazono et al.

Clinica chimica acta; international journal of clinical chemistry, 412(15-16), 1436-1440 (2011-05-10)

In human serum, as for phospholipids not containing choline, phosphatidylethanolamine (PE) exists approximately 5% in a whole phospholipid. PE is well known as one of the main components of biological membranes, and also plays important roles that contribute to apoptosis

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Articles

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protocols

Enzymatic Assay of Malic Dehydrogenase

Spectrophotometric assay evaluates malic dehydrogenase activity using bovine heart enzyme with critical histidine residue at active site.

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Malic Dehydrogenase from bovine heart