M9170

Sigma-Aldrich

Macrophage Colony-Stimulating Factor from mouse

M-CSF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):
mCSF-1, mM-CSF, CSF-1, M-CSF
CAS Number:
EC Number:
MDL number:
eCl@ss:
32190102

Quality Level

biological source

mouse

recombinant

expressed in E. coli

assay

≥98% (SDS-PAGE)

form

lyophilized powder

potency

0.500-3.000 ng/mL ED50

quality

endotoxin tested

mol wt

dimer 18.2 kDa (containing 156 amino acid residues)

packaging

pkg of 10 μg

application(s)

cell culture | mammalian: suitable

impurities

≤1.000 EU/μg

color

white

UniProt accession no.

storage temp.

−20°C

Gene Information

mouse ... Csf1(12977)

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Application

Macrophage Colony-Stimulating Factor from mouse has been used:
  • in the initiation of osteoclastogenesis in bone marrow monocyte cells
  • to stimulate osteoclast differentiation in non-adherent hematopoietic cell
  • for the generation of blood-derived stem cells

Biochem/physiol Actions

Macrophage Colony Stimulating Factor (M-CSF), also known as CSF-1, can be produced by a number of cells, including fibroblasts, monocytes, activated macrophages, secretory epithelial cells of the endometrium, endothelial cells activated by LPS or cytokines, and bone marrow stromal cells. In addition to its namesake activity, M-CSF can stimulate the proliferation of isolated macrophages, augment the production and release of cytokines and other inflammatory modulators from macrophages, enhance macrophage antibody-dependent cell-mediated cytotoxicity, prime and enhance macrophages in their ability to kill tumor cells and other microorganisms, stimulate pinocytosis, and support osteoclast differentiation. M-CSF binds and activates a 165 kDa glycoprotein of the receptor tyrosine kinase subclass III or the RTK subfamily. Due to alternative splicing of a single M-CSF gene and due to variations in glycosylation, the molecular weight of natural soluble M-CSF ranges from 44-86 kDa. Human and mouse M-CSF share approximately 80% sequence homology in the conserved N-terminal region of 150 amino acids. Although human M-CSF is active in murine systems, mouse M-CSF appears to be species-specific in its actions.

Physical form

Lyophilized from a sterile filtered buffered aqueous solution.

Analysis Note

The proliferative activity is tested by the dose-dependent stimulation of the murine monocytic cell line, M-NFS-60.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

hazcat

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Elisabeth A Diget et al.
Mediators of inflammation, 2013, 208412-208412 (2013-02-23)
Macrophages play an important role in human immunodeficiency virus (HIV) pathogenesis and contribute to establishment of a viral reservoir responsible for continuous virus production and virus transmission to T cells. In this study, we investigated the differences between various monocyte-derived...
B F Boyce
Journal of dental research, 92(10), 860-867 (2013-08-03)
Osteoclasts are derived from mononuclear hematopoietic myeloid lineage cells, which are formed in the bone marrow and are attracted to the bloodstream by factors, including sphingsine-1 phosphate. These circulating precursors are attracted to bone surfaces undergoing resorption by chemokines and...
Filamin A regulates monocyte migration through Rho small GTPases during osteoclastogenesis
Leung R, et al.
Journal of Bone and Mineral Research, 25(5), 1077-1091 (2010)
Monica R P Elmore et al.
Neuron, 82(2), 380-397 (2014-04-20)
The colony-stimulating factor 1 receptor (CSF1R) is a key regulator of myeloid lineage cells. Genetic loss of the CSF1R blocks the normal population of resident microglia in the brain that originates from the yolk sac during early development. However, the...
Noushine Mossadegh-Keller et al.
Nature, 497(7448), 239-243 (2013-04-12)
Under stress conditions such as infection or inflammation the body rapidly needs to generate new blood cells that are adapted to the challenge. Haematopoietic cytokines are known to increase output of specific mature cells by affecting survival, expansion and differentiation...

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