Preparing this product in a concentrated form is not recommended as some salt complexes may precipitate. Supplements may be added prior to sterilization or added aseptically to a sterile medium. Certain supplements (i.e. heat labile) may require filter sterilization and may affect the shelf life of the medium. The basic steps for preparing culture medium are the following: 1. Using a container twice the size of the desired final volume, measure out approximately 90% of the final required volume of tissue culture grade water (e.g. Sigma Product No. W-3500). Example: 900 ml for a final volume of 1000 ml. 2. While stirring, add the powdered product. 3. Rinse the original container with a small volume of tissue culture grade water to remove traces of the powder. Add to the solution in Step 2. 4. Add desired supplements (e.g. auxins, cytokinins). 5. While stirring, adjust to the desired pH (e.g. 5.7 +/- 0.1) using KOH, NaOH, or HCl. 6. Add additional tissue culture grade water to bring the medium to the final volume. 7. Heat the solution to approx. 100o C while stirring. 8. Dispense the medium into culture vessels before or after autoclaving according to your application. Add heat labile constituents after autoclaving. 9. Sterilize the medium in a validated autoclave at 1Kg/cm2 (15 psi). The medium should attain a temperature of 121o C for at least 15 min. Refer to the Sigma Plant Cell Culture Catalog for recommended autoclave times for different volumes.
M9274
Murashige and Skoog Basal Medium
suitable for plant cell culture, with sucrose and agar
Synonym(s):
MS Basal Medium
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Quality Level
form
powder
technique(s)
cell culture | plant: suitable
application(s)
agriculture
shipped in
ambient
storage temp.
2-8°C
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1 of 4
This Item | M0404 | M5519 | M7024 |
|---|---|---|---|
| application(s) agriculture | application(s) agriculture | application(s) agriculture | application(s) agriculture |
| form powder | form powder | form powder | form powder |
| technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in ambient | shipped in ambient | shipped in - | shipped in ambient |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Application
Preparation Note
Media Formulation
related product
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Ox. Sol. 3
Storage Class
5.1B - Oxidizing hazardous materials
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
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What are the steps to follow in preparing the culture medium for M9274 Basal medium?
1 answer-
Helpful?
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Would an incorrect pH level affect the setting of the agar?
1 answer-
Yes, gelation is pH dependent. Adjusting the pH to 5.7 is recommended prior to heating to melt the agar and autoclaving to sterilize. Please see the link below to review the general plant culture medium preparation instructions available in the datasheet for product M5519 (formulation without agar):
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/361/537/m5519pis.pdfHelpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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How is Murashige and Skoog Basal Medium, Product M9274 different from Product M5519?
1 answer-
M9274 contains sucrose at 30 g/L and agar at 8 g/L; M5519 does not.
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What is the stability of the prepared Murashige and Skoog Basal Medium, Product M9274?
1 answer-
This agar-containing medium can be stored for 3 to 6 months, depending on the stability of any additional supplements, if stored properly with no observable dehydration of the gel. If the medium appears to darken or pull away from the sides of the culture vessel, it should be discarded.
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How firm is the gel in the final sterile medium preparation of Murashige and Skoog Basal Medium, Product M9274?
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This formulation contains 8g/L of standard agar resulting in fairly firm and rigid culture medium.
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