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MAK003

Sigma-Aldrich

Branched Chain Amino Acid Kit

sufficient for 100 colorimetric tests

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UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

endocrinological disorders, diabetes; gastrointestinal diseases; neurological disorders

storage temp.

−20°C

Related Categories

General description

The branched-chain amino acids (BCAA), leucine, isoleucine, and valine, are the most common essential amino acids in proteins. The BCAA may function in both energy production and as nutrient signals. Dietary supplementation with BCAA may aid in the treatment of various disorders such as Amyotrophic Lateral Sclerosis (ALS), latent and chronic hepatic encephalopathies, and to prevent muscle atrophy in cancer patients. BCAA may increase the rate of protein synthesis and sensitize cells to insulin and insulin-like growth factor.

Application

Branched Chain Amino Acid Kit has been used to determine the concentration of hepatic branched-chain amino acids, in a study assessing the role of CREB regulated transcription coactivator 2 (CRTC2) in hepatic lipid homeostasis and obesity in mice.

Suitability

Suitable for measuring Branched Chain Amino Acids (BCAA′s) in a variety of biological samples.

Principle

In this assay, BCAA concentration is determined using a coupled enzyme reaction, which results in a colorimetric (450 nm) product, proportional to the BCAA present. The BCAA Assay Kit provides a simple convenient means of measuring the BCAAs in a variety of biological samples.

pictograms

Corrosion

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Danger

hcodes

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

wgk_germany

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Juliane Zemdegs et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 39(30), 5935-5948 (2019-06-05)
Epidemiological studies indicate that insulin resistance (IR), a hallmark of type 2 diabetes, is associated with an increased risk of major depression. Here, we demonstrated that male mice fed a high-fat diet (HFD) exhibited peripheral metabolic impairments reminiscent of IR
Natasha Fillmore et al.
American journal of physiology. Endocrinology and metabolism, 315(5), E1046-E1052 (2018-08-15)
Recent studies have proposed that elevated branched-chain amino acids (BCAAs) may induce insulin resistance (IR) in muscle secondary to increased BCAA oxidation inhibiting glucose oxidation (GO) and fatty acid oxidation (FAO). However, BCAA oxidation rates have not been assessed in
Wouter De Haes et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(24), E2501-E2509 (2014-06-04)
The antiglycemic drug metformin, widely prescribed as first-line treatment of type II diabetes mellitus, has lifespan-extending properties. Precisely how this is achieved remains unclear. Via a quantitative proteomics approach using the model organism Caenorhabditis elegans, we gained molecular understanding of
Multifaceted role of insulin-like growth factors and mammalian target of rapamycin in skeletal muscle.
Frost R A and Charles H L
Endocrinol. Metab. Clin., 41(2), 297-322 (2012)
Protein substitution to produce a processed cheese with high branched-chain amino acids of medical and genetic importance.
El-Shazly H A M, et al.
The Egyptian journal of medical human genetics., 11(2), 121-133 (2010)

Articles

Assay protocol for the colorimetric detection of Branched Chain Amino Acids (BCAA) in biological samples using the alanine assay kit.

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