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MAK078

Sigma-Aldrich

Xanthine Oxidase Activity Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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Synonym(s):
XO Activity Assay Kit
NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric
fluorometric

relevant disease(s)

rheumatological diseases; gastrointestinal diseases; cardiovascular diseases

storage temp.

−20°C

Gene Information

human ... XDH(7498)
mouse ... XDH(22436)
rat ... XDH(497811)

Related Categories

General description

Xanthine Oxidase (XO) catalyzes the sequential oxidation of hypoxanthine to xanthine, and xanthine to uric acid and hydrogen peroxide. In humans and other primates, XO controls the final step of purine catabolism and is normally found in the liver and the intestinal mucosa. In rodents, XO is broadly expressed in most tissues. While XO activity is normally very low in blood, liver injury can result in the release of XO into blood. XO may contribute to the pathogenesis of gout and cardiovascular disease, and XO activity or expression may be upregulated in these conditions.

Application

Xanthine Oxidase Activity Assay Kit has been used to measure the xanthine oxidase in serum samples.

Suitability

Suitable for the measurement of xanthine oxidase in a variety of samples including tissue, cells and serum

Principle

The Xanthine Oxidase Activity Assay Kit provides a simple and direct procedure for measuring XO activity in a variety of biological samples. XO activity is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the hydrogen peroxide generated. One unit of XO is defined as the amount of enzyme that catalyzes the oxidation of xanthine, yielding 1.0 μmole of uric acid and hydrogen peroxide per minute at 25 °C.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

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