MAK085

Sigma-Aldrich

Lipid Peroxidation (MDA) Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):
MDA Assay, TBARS Assay, Thiobarbituric Acid Reactive Substances Assay
NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

drug control

regulated under CDSA - not available from Sigma-Aldrich Canada

detection method

colorimetric
fluorometric

storage temp.

−20°C

Related Categories

General description

Lipid peroxidation is the degradation of lipids that occurs as a result of oxidative damage and is a useful marker for oxidative stress. Polyunsaturated lipids are susceptible to an oxidative attack, typically by reactive oxygen species, resulting in a well-defined chain reaction with the production of end products such as malondialdehyde (MDA). Lipid peroxidation may contribute to the pathology of many diseases including atherosclerosis, diabetes, and Alzheimer′s.

Application

Lipid peroxidation (MDA) assay kit has been used to determine the levels of malondialdehyde (MDA).

Suitability

Suitable for the measurement of malondialdehyde (MDA) in a variety of samples including tissue, cells and plasma

Principle

In this kit, lipid peroxidation is determined by the reaction of MDA with thiobarbituric acid (TBA) to form a colorimetric (532 nm)/fluorometric (λex = 532/λem = 553 nm) product, proportional to the MDA present.

Signal Word

Danger

Hazard Statements

RIDADR

UN 3316 9

Flash Point(F)

57.2 °F - closed cup

Flash Point(C)

14 °C - closed cup

Certificate of Analysis

Certificate of Origin

Aldehyde dehydrogenase 2 protects against oxidative stress associated with pulmonary arterial hypertension.
Xu T, et al.
Redox Biology, 11, 286-296 (2017)
Marco A Perrone et al.
Journal of cardiovascular medicine (Hagerstown, Md.), 20(7), 419-426 (2019-10-09)
Cardiovascular diseases (CVDs) are the most frequent causes of death in the world. Inflammation and oxidative damage contribute significantly to the development of atherosclerosis and CVDs. European Food Safety Authority scientific opinion has acknowledged that hydroxytyrosol (3,4-dihydroxyphenylethanol) and derivatives, contained...
Charlotte Berthelot et al.
International journal of phytoremediation, 19(12), 1118-1125 (2017-05-20)
Dark septate endophytes (DSEs) are abundant in stressful environments, including trace element (TE)-enriched soils. However, knowledge about the effects of DSEs on plant growth in such soils is poor compared to the well-known mycorrhizal fungi. The aim of this work...
Karin Wendin et al.
Foods (Basel, Switzerland), 9(6) (2020-06-21)
To increase the acceptability of insects as food in Western culture, it is essential to develop attractive, high-quality food products. Higher acceptability of insect-based food has been shown if the insects are "invisible". Mincing or chopping the insect material could...
Maria I Ayuso et al.
ACS chemical neuroscience, 8(10), 2202-2213 (2017-07-22)
There is a need to develop additional effective therapies for ischemic stroke. Nitrones, which were first developed as reactive oxygen species (ROS)-trapping compounds, have been proposed as neuroprotective agents for ischemic stroke, a ROS-related disorder. The previous reported ROS-trapping compound...

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