MAK119

Sigma-Aldrich

Acetylcholinesterase Activity Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):
RBC Cholinesterase Activity Assay, ACHE Activity Assay
NACRES:
NA.84
Pricing and availability is not currently available.

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

storage temp.

room temp

Gene Information

human ... ACHE(43)
mouse ... ACHE(11423)

Related Categories

General description

Acetylcholinesterases (AChEs) are enzymes that hydrolyze the neurotransmitter acetylcholine (ACh) to acetate and choline. AChE is located at the synaptic cleft and functions to terminate synaptic transmission by catalyzing the breakdown of ACh allowing cholinergic neurons to return to a resting state after activation. It is also found in membranes of red blood cells, motor and sensory fibers, muscles, nerves and central and peripheral tissues. Changes in AChE activity may result from exposure to certain insecticides, which act as cholinesterase inhibitors. Inhibitors of AChE are also used to treat certain conditions such as dementia.

Application

Acetylcholinesterase activity assay kit has been used to determine the activity of acetylcholinesterase in a rat organophosphate model and in brain tissue homogenates.

Features and Benefits

Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.

Suitability

Suitable for the detection of acetylcholinesterase activity in biological samples such as blood, serum, and plasma.

Principle

This assay is an optimized version of the Ellman method in which thiocholine, produced by AChE, reacts with 5,5′-dithiobis(2-nitrobenzoic acid) to form an colorimetric (412 nm) product, proportional to the AChE activity present. One unit of AChE is the amount of enzyme that catalyzes the production of 1.0 μmole of thiocholine per minute at room temperature at pH 7.5. This kit has a linear range of 10-600 units/L of AChE activity.

Hazard Codes

Xn

Risk Statement

22-36/37/38

Safety Statement

26

RIDADR

NONH for all modes of transport

Certificate of Analysis
Certificate of Origin
Study on social isolation as a risk factor in development of Alzheimer?s disease in rats.
Ali A A, et al.
Brain disorders & therapy., 6(230), 2-2 (2017)
Zhen-Xing Zuo et al.
Neural plasticity, 2015, 453170-453170 (2015-12-24)
Chronic pain is a major health issue and most patients suffer from spontaneous pain. Previous studies suggest that Huperzine A (Hup A), an alkaloid isolated from the Chinese herb Huperzia serrata, is a potent analgesic with few side effects. However...
Neuroprotective Effects of AEOL10150 in a Rat Organophosphate Model.
Liang L P, et al.
Toxicological Sciences, 162(2), 611-621 (2017)
Marine Droguerre et al.
Frontiers in neuroscience, 14, 563-563 (2020-07-03)
Donepezil (DPZ) is an acetylcholinesterase inhibitor used in Alzheimer's disease to restore cognitive functions but is endowed with limited efficacy. Recent studies pointed out the implication of astroglial networks in cognitive processes, notably via astrocyte connexins (Cxs), proteins involved in...
P Kumari et al.
Parasitology, 144(10), 1412-1416 (2017-06-07)
Demodex canis infestation in dogs remains one of the main challenges in veterinary dermatology. The exact pathogenesis of canine demodicosis is unknown but an aberration in immune status is considered very significant. No studies have underpinned the nexus between induction...

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