MAK119

Sigma-Aldrich

Acetylcholinesterase Activity Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):
RBC Cholinesterase Activity Assay, ACHE Activity Assay
NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

storage temp.

room temp

Gene Information

human ... ACHE(43)
mouse ... ACHE(11423)

Related Categories

General description

Acetylcholinesterases (AChEs) are enzymes that hydrolyze the neurotransmitter acetylcholine (ACh) to acetate and choline. AChE is located at the synaptic cleft and functions to terminate synaptic transmission by catalyzing the breakdown of ACh allowing cholinergic neurons to return to a resting state after activation. It is also found in membranes of red blood cells, motor and sensory fibers, muscles, nerves and central and peripheral tissues. Changes in AChE activity may result from exposure to certain insecticides, which act as cholinesterase inhibitors. Inhibitors of AChE are also used to treat certain conditions such as dementia.

Application

Acetylcholinesterase activity assay kit has been used to determine the activity of acetylcholinesterase in a rat organophosphate model and in brain tissue homogenates.

Features and Benefits

Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.

Suitability

Suitable for the detection of acetylcholinesterase activity in biological samples such as blood, serum, and plasma.

Principle

This assay is an optimized version of the Ellman method in which thiocholine, produced by AChE, reacts with 5,5′-dithiobis(2-nitrobenzoic acid) to form an colorimetric (412 nm) product, proportional to the AChE activity present. One unit of AChE is the amount of enzyme that catalyzes the production of 1.0 μmole of thiocholine per minute at room temperature at pH 7.5. This kit has a linear range of 10-600 units/L of AChE activity.

RIDADR

NONH for all modes of transport

Abdullah M Alzahrani
Environmental science and pollution research international, 26(16), 16548-16555 (2019-04-14)
Despite the widespread use of the insecticide imidacloprid (IMI), a neonicotinoid, there is an urgent need for documenting information related to its acute toxicity. Therefore, this study aims to explore the markers of IMI acute toxicity in the testes of...
Study on social isolation as a risk factor in development of Alzheimer?s disease in rats.
Ali A A, et al.
Brain disorders & therapy., 6(230), 2-2 (2017)
Amrita Mukherjee et al.
Environmental science and pollution research international, 26(23), 23442-23452 (2019-06-15)
The current study demonstrates oxidative damage and associated neurotoxicity following pH stress in two freshwater carp Labeo rohita and Cirrhinus cirrhosus. Carp (n = 6, 3 replicates) were exposed to four different pH (5.5, 6, 7.5, and 8) against control (pH 6.8 ± 0.05) for...
P Kumari et al.
Parasitology, 144(10), 1412-1416 (2017-06-07)
Demodex canis infestation in dogs remains one of the main challenges in veterinary dermatology. The exact pathogenesis of canine demodicosis is unknown but an aberration in immune status is considered very significant. No studies have underpinned the nexus between induction...
Marine Droguerre et al.
Frontiers in neuroscience, 14, 563-563 (2020-07-03)
Donepezil (DPZ) is an acetylcholinesterase inhibitor used in Alzheimer's disease to restore cognitive functions but is endowed with limited efficacy. Recent studies pointed out the implication of astroglial networks in cognitive processes, notably via astrocyte connexins (Cxs), proteins involved in...

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