The principle of this kit is the reaction of formaldehyde with acetoacetanilide in the presence of ammonia. Neither a MESNA (4-Morpholineethanesulfonic acid, sodium) buffer nor EDTA in the test sample would be expected to interfere with this reaction. Having said that, it would be appropriate to run a blank containing MESNA and EDTA, and standards spiked with MESNA and EDTA, to confirm they they are not interfering with the test result...that is, making it either artificially high or low.
MAK131
Formaldehyde Assay
sufficient for 100 fluorometric tests
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1 KIT
$460.00
About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
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usage
sufficient for 100 fluorometric tests
detection method
fluorometric
relevant disease(s)
cancer
storage temp.
2-8°C
Related Categories
1 of 4
This Item | MAK321 | 1.14678 | 252549 |
|---|---|---|---|
| detection method fluorometric | detection method fluorometric | detection method photometric (Chromotropic acid) | detection method - |
| usage sufficient for 100 fluorometric tests | usage sufficient for 100 fluorometric tests | usage sufficient for 100 tests | usage - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 15-25°C | storage temp. - |
| relevant disease(s) cancer | relevant disease(s) gastrointestinal diseases | relevant disease(s) - | relevant disease(s) - |
General description
Formaldehyde is widely employed in industry for a wide range of applications. It acts as a disinfectant and biocide, and is utilized as a tissue fixative and embalming agent.[1] Formaldehyde is a key metabolite produced during normal metabolism and contributes to methylation reactions and protein and nucleic acid synthesis. Increased production of formaldehyde has been observed in many cancer cell lines and increased formaldehyde concentration in urine has been associated with bladder and prostrate cancer.[2]
Application
- Modified Natural Rubber as a Simple Chemical Sensor with Smartphone Detection for Formaldehyde Content in a Seafood Sample.: This article explores the use of modified natural rubber as a chemical sensor, paired with smartphone detection, to measure formaldehyde content in seafood. This approach highlights the potential for accessible and mobile detection systems in food safety applications (Yeerum et al., 2022).
- Optical enzymatic formaldehyde biosensor based on alcohol oxidase and pH-sensitive methacrylic-acrylic optode membrane.: The study develops an optical enzymatic biosensor for formaldehyde, using alcohol oxidase with a pH-sensitive membrane, offering a novel approach for quick and sensitive detection in various analytical applications (Nurlely et al., 2022).
Features and Benefits
Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.
Suitability
Suitable for the detection of formaldehyde in urine and other biological samples.
Principle
The Formaldehyde Assay kit provides a simple and direct procedure for measuring formaldehyde levels in urine and other biological samples. In this assay, formaldehyde is derivatized with acetoacetanilide in the presence of ammonia resulting in a fluorescent (λex = 370/λem = 470 nm) product, proportional to the formaldehyde present.
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 1 - STOT SE 3
target_organs
Eyes,Central nervous system, Respiratory system
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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Is the assay compatible with MES buffer? Is EDTA affecting the reaction?
1 answer-
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What is the limit of detection that the MAK131 Formaldehyde Assay is capable of?
1 answer-
The kit is capable of detecting formaldehyde down to 1.5 micromolar or 45 ppb. The upper end of detection for undiluted samples is 100 micromolar, which is the highest concentration of the standard curve.
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