The kit instructions are published in the product datasheet. This document is located in the 'DOCUMENTATION' section under 'More Documents'. Please use the links below to access the datasheet and the documentation section of the product page:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/138/239/mak137bul.pdf
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MAK137
Phospholipase D Activity Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Synonym(s):
PLD Activity Assay Kit
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usage
sufficient for 100 colorimetric or fluorometric tests
detection method
colorimetric, fluorometric
relevant disease(s)
cancer
storage temp.
−20°C
Gene Information
human ... PLD1(5337), PLD2(5338), PLD3(23646), PLD4(122618), PLD5(200150), PLD6(201164)
mouse ... PLD1(18805), PLD2(18806), PLD3(18807), PLD4(104759), PLD5(319455), PLD6(194908)
rat ... PLD1(25096), PLD2(25097), PLD3(361527), PLD4(362792), PLD5(289270), PLD6(287366)
Related Categories
1 of 4
This Item | |||
|---|---|---|---|
| Gene Information human ... PLD1(5337), PLD2(5338), PLD3(23646), PLD4(122618), PLD5(200150), PLD6(201164) | Gene Information human ... PLD1(5337) | Gene Information human ... PLD2(5338) | Gene Information human ... PLD1(5337) |
| detection method colorimetric, fluorometric | detection method - | detection method - | detection method - |
| usage sufficient for 100 colorimetric or fluorometric tests | usage - | usage - | usage - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| relevant disease(s) cancer | relevant disease(s) - | relevant disease(s) - | relevant disease(s) - |
General description
Phospholipase D (PLD) catalyzes the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and choline. It is ubiquitously expressed and is implicated in immune function and cancer inhibition. Abnormalities in PLD expression have been associated with human cancers such as breast, renal, gastric and colorectal cancer.
Application
Phospholipase D (PLD) Activity Assay Kit has been used to measure PLD activity in the liver and lysosomal fractions of the mice liver.[1]
Suitable for the detection of phospholipase D activity in biological samples. This kit can be used to study the effects of various compounds on phospholipase D activity.
Suitable for the quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism.
Biochem/physiol Actions
In this assay, PLD hydrolyzes phosphatidylcholine to choline, which is determined using choline oxidase resulting in a colorimetric (570 nm)/fluorometric (λex = 530/λem = 585 nm) product, proportional to the PLD activity in the sample. Unit definition: one unit of PLD catalyzes the formation of 1 mmole choline per minute under the assay conditions (pH 7.4).
Features and Benefits
Compatible with high-throughput handling systems.
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - Resp. Sens. 1
Storage Class
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
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Phospholipase D.
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Related Content
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Where are the Instructions for use, please?
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Where are the instructions for the use of the Phospholipase D assay kit, please?
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The Protocol for this kit is available in the product datasheet. Please see the link below to access this document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/138/239/mak137bul.pdf
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Can the Phospholipase D Activity Assay Kit and the PLC Activity Kits be used in Human whole blood? Thanks,
1 answer-
The Phospholipase D Activity Assay Kit is suitable for use with biological samples, however whole blood has not been tested. As this is a colorimetric reaction, the resulting pink color would very likely be masked. Serum or plasma would be recommended.
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