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MilliporeSigma

MAK138

Autophagy Assay Kit

sufficient for 200 fluorometric tests

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$355.00

$355.00

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About This Item

NACRES:
NA.84
UNSPSC Code:
12161503

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Product Name

Autophagy Assay Kit, sufficient for 200 fluorometric tests

usage

sufficient for 200 fluorometric tests

detection method

fluorometric

relevant disease(s)

cancer; immunological diseases

storage temp.

−20°C

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Autophagy Assay Kit sufficient for 200 fluorometric tests

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detection method

fluorometric

detection method

fluorometric

detection method

colorimetric, fluorometric

detection method

fluorometric

usage

sufficient for 200 fluorometric tests

usage

sufficient for 100 fluorometric tests

usage

sufficient for 100 colorimetric or fluorometric tests

usage

sufficient for 100 fluorometric tests

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

relevant disease(s)

cancer; immunological diseases

relevant disease(s)

endocrinological disorders, diabetes; neurological disorders; cancer

relevant disease(s)

gastrointestinal diseases; cancer

relevant disease(s)

gastrointestinal diseases; cardiovascular diseases

Application


  • Inhibitory Effect of Ursolic Acid on Proliferation and Migration of Renal Carcinoma Cells and Its Mechanism.: This study explores the inhibitory effects of ursolic acid on the proliferation and migration of renal carcinoma cells. The use of an autophagy assay kit was instrumental in demonstrating that ursolic acid induces autophagy, leading to decreased cell viability and migration. These findings highlight the potential of ursolic acid as a therapeutic agent in renal carcinoma through the modulation of autophagy (Lyu et al., 2022).

This kit is suitable for the detection of autophagy in mammalian cells by fluorescence microscopy and fluorescence microplate readers.

Biochem/physiol Actions

The Autophagy Assay kit provides a simple and direct procedure for measuring autophagy in a variety of cell types using a proprietary fluorescent autophagosome marker (λex = 333/λem = 518 nm).

General description

Autophagy is an evolutionarily conserved degradation process that targets long-lived proteins, organelles, and other cytoplasmic components for degradation via the lysosomal pathway. Activation of the autophagy pathway is required for multiple cellular roles, including survival during starvation, the clearance of intracellular components, development, and immunity.[1][2]

Storage Class

10 - Combustible liquids

wgk

WGK 2

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
3760901
3760901
3510893
3320403
3041403

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Autophagy: machinery and regulation.
Yin Z, et al.
Microbial cell, 3(12), 588-588 (2016)
Ilenia Martinelli et al.
Redox biology, 40, 101866-101866 (2021-01-26)
Autophagy and apoptosis are powerful regulators of multiple facets of cellular metabolism and homeostasis. Here, we uncover that galanin, a pleiotropic peptide, regulates cardiac autophagy and deactivates apoptotic cell death through the Forkhead box protein O1 (FoxO1) pathway. In hypertrophied
Autophagy in the pathogenesis of disease.
Levine B and Guido K
Cell, 132(1), 27-42 (2008)
Aneta Rogalska et al.
PloS one, 13(7), e0201296-e0201296 (2018-07-25)
Chemical modification of known, effective drugs are one method to improve the chemotherapy of tumors. We reported ability of oxazoline analogs of doxorubicin (O-DOX) and daunorubicin (O-DAU) to induce apoptosis and autophagy in ovarian and liver cancer cells. Reactive oxygen
Simin Nazarnezhad et al.
Neurotoxicology, 74, 196-202 (2019-07-20)
Diabetic neuropathy (DN) is the most common complication of diabetes mellitus. It is thought that neuronal cell death which is mainly due to reactive oxygen species (ROS) overproduction in the cells is responsible for most symptoms of this disorder. Nesfatin-1
View All Related Papers

Questions

1–5 of 5 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. if I have cells expressing GFP, Can I detect autophagy without the signal overlapping?

    1 answer

    1. This kit operates with an λex = 333/λem = 518 nm as the probe in this kit is a blue fluorescent dye. It is our understanding that GFP has an excitation of 395 nm with an emission at 508 nm. It is unclear if there is any signal overlap and this study would need to be determined empirically in a laboratory setting.

      Helpful?

  3. The autophagy assay kit MAK138 says that it "measures autophagy by using a proprietary fluorescent autophagosome marker" However, it is not clear as to what is this marker? Where does this marker bind on the autophagosome? Does it bind to LC3II?

    1 answer

    1. The Autophagosome Detection Reagent used in the kit is a fluorescence marker that can label the acidic autophagosomes. It can be accumulated in autophagic vacuoles via both ion trapping and specific interactions with vacuole membrane lipids.

      Helpful?

  4. Can this autophagy assay kit MAK138 detect whether autophagosome has merged with lysosome? Can this assay distinguish between autophagy initiation and autophagy completion?

    1 answer

    1. The kit can detect whether an autophagosome has merged with a lysosome. The kit cannot distinguish between autophagy initiation and autophagy completion.

      Helpful?

  5. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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