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MilliporeSigma

MAK142

Sigma-Aldrich

Fluorometric Intracellular Ros Kit

sufficient for 200 fluorometric tests (Deep red)

Synonym(s):

Intracellular ROS Fluorescence Assay

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1 KIT
$333.00

About This Item

EC Number:
200-664-3
UNSPSC Code:
12161503
NACRES:
NA.25

Key Documents

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$333.00

Usually ships in 2 weeks.
Request a Bulk Order
Technical Service
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usage

sufficient for 200 fluorometric tests (Deep red)

application(s)

cosmetics
food and beverages

detection method

fluorometric

relevant disease(s)

neurological disorders; cancer

storage temp.

−20°C

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Fluorometric Intracellular Ros Kit sufficient for 200 fluorometric tests (Deep red)

MAK142

Fluorometric Intracellular Ros Kit

Fluorometric Intracellular Ros Kit sufficient for 200 fluorometric tests (orange)

MAK144

Fluorometric Intracellular Ros Kit

Fluorometric Intracellular Ros Kit sufficient for 200 fluorometric tests (red)

MAK145

Fluorometric Intracellular Ros Kit

Fluorimetric Hydrogen Peroxide Assay Kit sufficient for 500 fluorometric tests (red fluorescence)

MAK165

Fluorimetric Hydrogen Peroxide Assay Kit

detection method

fluorometric

detection method

fluorometric

detection method

fluorometric

detection method

fluorometric

usage

sufficient for 200 fluorometric tests (Deep red)

usage

sufficient for 200 fluorometric tests (orange)

usage

sufficient for 200 fluorometric tests (red)

usage

sufficient for 500 fluorometric tests (red fluorescence)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

application(s)

cosmetics
food and beverages

application(s)

cosmetics
food and beverages

application(s)

-

application(s)

-

relevant disease(s)

neurological disorders; cancer

relevant disease(s)

cancer; neurological disorders

relevant disease(s)

neurological disorders; cancer

relevant disease(s)

cardiovascular diseases; aging/geriatric diseases; orthopedic diseases; pulmonary disorders; neurological disorders

General description

Reactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell. At physiological levels, ROS contribute to cell signaling and host defense.[1] Increased ROS generation, above the detoxification capacity of the biological system, results in oxidative stress and cellular damage.[2] The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA. ROS has been implicated in disease states, such as Alzheimer′s disease, Parkinson′s disease, cancer, and aging.[3]

Application


  • Diethyl Blechnic Exhibits Anti-Inflammatory and Antioxidative Activity via the TLR4/MyD88 Signaling Pathway in LPS-Stimulated RAW264.7 Cells.: This article uses fluorometric methods to explore the anti-inflammatory and antioxidative properties of diethyl blechnic, illustrating the role of ROS assays in elucidating the molecular mechanisms underlying the therapeutic effects of natural compounds (Yang S et al., 2019).

Suitability

This kit is suitable for the detection of intracellular ROS by fluorescence microplate reader, flow cytometer, or a fluorescent microscope with TRITC filter.

Principle

The Fluorometric Intracellular ROS Kit provides a sensitive, one-step fluorometric assay to detect intracellular ROS (especially superoxide and hydroxyl radicals) in live cells after 1 hour incubation. ROS react with a fluorogenic sensor localized to the cytoplasm, resulting in a fluorometric product proportional to the amount of ROS present. The assay can be performed in either a 96 or 384 multiwell plate format and with detection at λex = 650/λem = 675 nm.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
3510351
3181881
2781712
3040552
259113

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Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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