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MAK166

Sigma-Aldrich

Fluorimetric Hydrogen Peroxide Assay Kit

sufficient for 500 fluorometric tests (infrared fluorescence)

EC Number:
NACRES:
NA.84

usage

sufficient for 500 fluorometric tests (infrared fluorescence)

detection method

fluorometric

storage temp.

−20°C

General description

Hydrogen peroxide, a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration.

Application

Fluorimetric Hydrogen Peroxide Assay Kit has been sued to determine the oxidation pressure (H2O2).

Features and Benefits

This assay is compatible with high-throughput handling systems.

Suitability

The Fluorescent Hydrogen Peroxide Assay Kit provides a simple and reproducible method to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions.

Principle

Fluorimetric Hydrogen Peroxide Assay Kit can also be used to detect hydrogen peroxide released from living cells or produced by oxidase activities. This kit utilizes a peroxidase substrate that generates an infrared fluorescent product (λex = 640/λem = 680 nm) after reaction with hydrogen peroxide that can be analyzed by a fluorescent microplate reader. The generation of the fluorescence product is pH independent between pH 4 to pH 10 and is superior to other fluorescence kits for measuring hydrogen peroxide in low pH solutions which often display decreased probe sensitivity. The infrared fluorescent probe is less sensitive to assay background due to autofluorescence from biological samples. This assay is compatible with high-throughput handling systems.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Product Information Sheet

Quotes and Ordering

Guixi Gao et al.
Cell chemical biology, 24(7), 881-891 (2017-07-18)
Bacterial aromatic polyketides are a group of natural products synthesized by polyketide synthases (PKSs) that show diverse structures and biological activities. They are structurally subclassified into linear, angular, and discoid aromatic polyketides, the formation of which is commonly determined by
Fritz Schweikart et al.
Analytical chemistry, 91(3), 1748-1751 (2019-01-24)
HPLC coupled to both UV and MS is an established setup for purity assessments in many areas. With evolving technology, instrument sensitivity increases, calling for lower sample concentration, while light flux in a commercial UV detector cell is considerably higher
The mechanism of iron-compensation for manganese deficiency of Streptococcus pneumoniae.
Cao K, et al.
Journal of Proteomics, 184, 62-70 (2018)
Formation of an angular aromatic polyketide from a linear anthrene precursor via oxidative rearrangement.
Gao G, et al.
Cell Chemical Biology, 24(7), 881-891 (2017)
Alexander Jonathan Spitzer et al.
Oxidative medicine and cellular longevity, 2020, 3894309-3894309 (2020-04-11)
The aim of this study was to investigate the mechanisms underlying the reduced milk production during mastitis. We hypothesized that bacterial endotoxin induces hypoxia, oxidative stress, and cell apoptosis while inhibiting milk gene expression in the mammary gland. To test

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