MilliporeSigma
All Photos(2)

MAK198

Sigma-Aldrich

2-Phosphoglycerate Assay Kit

Sufficient for 100 Colorimetric or Fluorometric tests

All Photos(2)
Synonym(s):
2-PG Assay Kit, 2PG Assay Kit
NACRES:
NA.84

detection method

colorimetric
fluorometric

storage temp.

−20°C

Related Categories

Compare Similar Items

View Full Comparison

1 of 4

This Item
MAK439MAK168MAK122
2-Phosphoglycerate Assay Kit Sufficient for 100 Colorimetric or Fluorometric tests

Sigma-Aldrich

MAK198

2-Phosphoglycerate Assay Kit

Glycolysis Assay Kit Sufficient for 100 colorimetric tests

Sigma-Aldrich

MAK439

Glycolysis Assay Kit

Pyrophosphate Assay Kit sufficient for 200 fluorometric tests (Blue fluorescence)

Sigma-Aldrich

MAK168

Pyrophosphate Assay Kit

Phospholipid Assay Kit sufficient for 100 colorimetric or fluorometric tests

Sigma-Aldrich

MAK122

Phospholipid Assay Kit

detection method

colorimetric, fluorometric

detection method

colorimetric

detection method

fluorometric

detection method

colorimetric, fluorometric

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

2-Phosphoglycerate (2PG) is an important intermediate in the glycolysis pathway, a critical mechanism of ATP generation. It is converted to phosphoenol pyruvate (PEP), catalyzed by the enzyme enolase. The consumption of glucose and the rate of glycolysis is upregulated in metastatic cancers, a phenomenon known as the Warburg effect. Measurement of intracellular levels of 2PG is a tool for analyzing the glycolytic pathway in cancer cells.

Suitability

Suitable for the detection of 2-Phosphoglycerate in tissue and cell culture samples.

Principle

The 2-Phosphoglycerate Colorimetric/Fluorometric Assay Kit is a simple assay that measures 2PG in various samples (ranging from 2-10 nmole/well for the colorimetric assay and from 50-250 pmole/well for the fluorometric assay). 2PG concentration is determined by converting the 2PG to PEP and then to pyruvate. The pyruvate is oxidized, resulting in a colorimetric (570 nm) or fluorometric (λex = 535/ λem = 587 nm) product proportional to the amount of 2PG present.

Other Notes

The fluorometric assay is 10-100 times more sensitive than the colorimetric assay.

Kit Components Only

Product No.
Description
  • 2PG Assay Buffer
  • 2PG Probe, in DMSO
  • 2PG Enzyme Mix
  • 2PG Converter
  • 2PG Developer
  • 2PG Standard

Storage Class Code

12 - Non Combustible Liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

(enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service