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MAK246

Sigma-Aldrich

Neutrophil Elastase Activity Assay Kit (Fluorometric)

sufficient for 100 fluorometric tests

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NACRES:
NA.25

detection method

fluorometric

relevant disease(s)

hematological disorder; pulmonary disorders

storage temp.

−20°C

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Neutrophil Elastase Activity Assay Kit (Fluorometric) sufficient for 100 fluorometric tests

Sigma-Aldrich

MAK246

Neutrophil Elastase Activity Assay Kit (Fluorometric)

Urease Activity Assay Kit sufficient for 100 colorimetric tests

Sigma-Aldrich

MAK120

Urease Activity Assay Kit

Urokinase Activity Fluorometric Assay Kit Sufficient for 100 Fluorometric tests

Sigma-Aldrich

MAK185

Urokinase Activity Fluorometric Assay Kit

Plasmin Activity Assay Kit (Fluorometric) sufficient for 100 fluorometric tests

Sigma-Aldrich

MAK244

Plasmin Activity Assay Kit (Fluorometric)

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

General description

Neutrophil Elastase (NE, EC 3.4.21.37, leukocyte elastase, ELANE, ELA2, elastase 2, neutrophil elaszym, or serine elastase) is a cytotoxic serine protease. It is stored in the azurophil granules of neutrophil granulocyte and is released following cell stimulation, e.g. by pathogens, immune complexes or chemotactic agents (PMA). Mutation in the gene ELA2 (neutrophil elastase) results in neutropenia, a hereditary disorder characterized by reduced neutrophil count in an individual.

Suitability

Suitable to determine the activity of purified Neutrophil Elastase, detect the activity of Neutrophil Elastase in plasma and/or blood and screen/study/characterize chemotactic agents causing Neutrophil stimulation and release of Elastase.

Principle

The Neutrophil Elastase Activity Assay Kit utilizes the ability of NE to proteolytically cleave a synthetic substrate and release a fluorophore, AFC, which can be easily quantified by fluorescence. This assay kit is simple, rapid, and can detect as low as 1 ng of neutrophil elastase in a variety of samples.

Pictograms

Exclamation markHealth hazard
GHS07,GHS08

Signal Word

Danger

Hazard Statements

H315 - H319 - H334 - H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

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Neutrophil elastase in cyclic and severe congenital neutropenia.
Horwitz M S, et al.
Blood, 109(5), 1817-1824 (2007)
Sang-Il Han et al.
Bioresource technology, 302, 122791-122791 (2020-01-26)
The effect of co-cultivation of Porphyridium cruentum UTEX 161 with Pseudoalteromonas sp. MEBiC 03485 on P. cruentum growth and its sulfated polysaccharide (EPS) production were examined. The strain MEBiC 03485 had beneficial effects on P. cruentum growth, EPS production, and

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