This product has been discontinued without a replacement. Technical Service has no data describing its use in testing cell lysates.
MAK255
Cysteine Assay Kit (Fluorometric)
sufficient for 100 fluorometric tests
Synonym(s):
Cysteine Quantitation Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
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detection method
fluorometric
relevant disease(s)
cardiovascular diseases
storage temp.
−20°C
1 of 3
This Item | MAK151 | MAK173 |
|---|---|---|
| detection method fluorometric | detection method fluorometric | detection method fluorometric |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| relevant disease(s) cardiovascular diseases | relevant disease(s) endocrinological disorders, diabetes; cancer | relevant disease(s) endocrinological disorders, diabetes; neurological disorders; cardiovascular diseases; cancer |
General description
Cysteine (CYS) is a non-essential, sulfur containing amino acid in humans, related to cystine. Cysteine is important for protein synthesis, detoxification, and diverse metabolic functions. It is found in beta-keratin, which comprises the main protein in nails, skin, and hair. Cysteine is also important in collagen production, as well as skin elasticity and texture. A component of the antioxidant, glutathione, cysteine plays a role in the metabolism of essential biochemicals such as coenzyme A, heparin, and biotin. An elevated level of total cysteine can predict cardiovascular disease and metabolic syndromes. Cysteine deficiency is known to be one of the consequences of aging.[1]
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for the detection of cysteine in serum, plasma, and other biological fluids.
Principle
The Cysteine Assay Kit detects the physiological concentration of cysteine in a variety of biological fluids. The assay is based on the cleavage of thiol group of reduced cysteine generating a fluorometric product (ex = 365 nm/ em = 450 nm), directly proportional to the amount of total cysteine in the sample. The assay is specific and other thiol-based amino acids do not interfere with the assay. This high-throughput adaptable assay kit is simple and sensitive enough to detect to 10 µM of cysteine in a variety of samples.
Storage Class
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
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is this product suitable for cell lysate? If possible, i need protocol to prepare sample(cell number, how to lysis etc.)
1 answer-
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Could N-acetyl Cysteine interfere with the MAK255-1KT Cysteine Assay Kit (Fluorometric)? Additionally, would residual H2S also interfere with the assay?
1 answer-
Since the assay principle is based on the cleavage of the thiol group in reduced Cysteine, N-acetyl Cysteine, which contains the thiol group, is likely to be included in the quantification of cysteine present in the biological fluids by the kit. Additionally, the assay should detect Hydrogen sulfide generated by the enzymatic metabolism of Cysteine. Therefore, sulfide salts (such as Sodium sulfide) as well as residual Hydrogen sulfide in the sample would likely be a significant source of interference.
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Are the standards run in kinetic or endpoint mode? How are they plotted?
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The standards are run in kinetic mode as well. It's important that the delta T (T2-T1) remains consistent for both the standards and the samples, and in all cases, it should fall within the linear range of the plot. For instance, samples could be read from T=2 to T=4 (assuming linearity) and the standards could be read from T=3 to T=5 (again, assuming linearity), ensuring that the delta T remains the same. Subsequently, the delta RFU is plotted for the standards against concentration, and then the samples can be plotted using their delta RFU to determine the concentration.
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Is the product Cysteine Assay Kit (Fluorometric) MAK255 suitable for use with blood plasma?
1 answer-
The Cysteine Assay Kit (Fluorometric) MAK255 is suitable for use with mammalian plasma samples. Centrifugation of plasma samples is not necessary, as they can be used directly. While the choice of anticoagulant would not make a significant difference, it is generally recommended to avoid EDTA or citrate, which are chelators, and to use heparin instead.
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