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MAK309

Sigma-Aldrich

Bile Acid Assay Kit

sufficient for 100 fluorometric tests

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NACRES:
NA.84

detection method

fluorometric

relevant disease(s)

gastrointestinal diseases; cancer; cardiovascular diseases

storage temp.

−20°C

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This Item
MAK1264830570168
Bile Acid Assay Kit sufficient for 100 fluorometric tests

Sigma-Aldrich

MAK309

Bile Acid Assay Kit

Bilirubin Assay Kit sufficient for 180 colorimetric tests

Sigma-Aldrich

MAK126

Bilirubin Assay Kit

Bile salts suitable for microbiology

Millipore

48305

Bile salts

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

10-30°C

storage temp.

-

relevant disease(s)

gastrointestinal diseases; cancer; cardiovascular diseases

relevant disease(s)

hematological disorder; gastrointestinal diseases

relevant disease(s)

-

relevant disease(s)

-

General description

Twelve different types of bile acids are typically found in mammals, among them two primary types are cholic acid and chenodeoxycholic acid. These can be dehydroxylated into secondary bile acids. Finally, these four can be conjugated to either taurine or glycine creating 8 different conjugated bile acids. Bile acid levels in feces, blood, urine, and bile can be used as markers for various diseases such as hyperlipidemia, cholestasis, gall stones, colon cancer, etc. Bile acids also exist as sulfate salt forms known as bile acid sulfates. Sulfation of bile acids increases their solubility and decreases intestinal absorption, thereby enhancing fecal and urinary excretion.

Application

Bile Acid Assay Kit has been used to quantify bile acid levels from plasma.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the quantitative determination of total bile acids and evaluation of drug effects on bile acid metabolism in serum, plasma, urine and other biological samples.

Principle

This Bile Acid Assay Kit provides a convenient fluorimetric means to measure total bile acids in biological samples. In the assay, 3–hydroxysteroid dehydrogenase reacts with all twelve bile acids, converting NAD to NADH, which reduces a probe to a highly fluorescent product. The resulting fluorescence intensity (γex = 530 nm/γem = 585 nm) is linear to the bile acid concentration in the sample.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2


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Customers Also Viewed

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Bile acid transporters and regulatory nuclear receptors in the liver and beyond.
Halilbasic E, et al.
Journal of Hepatology, 58(1), 155-168 (2013)
Bile acids: chemistry, physiology, and pathophysiology.
Monte M J, et al.
World Journal of Gastroenterology, 15(7), 804-804 (2009)
Tarek Besheer et al.
The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology, 29(3), 299-307 (2018-05-15)
Chronic hepatitis C (CHC)-related mortality generally results from cirrhosis and subsequent complications. We aimed to investigate the potential role of plasma bile acid levels and ABCB11 1331T > C (V444A, rs2287622) (ATP-binding cassette subfamily B, member 11) gene polymorphism in
Ahmed Dawood Mohammed et al.
Nature communications, 13(1), 525-525 (2022-01-28)
Mucosal antibodies maintain gut homeostasis by promoting spatial segregation between host tissues and luminal microbes. Whether and how mucosal antibody responses influence gut health through modulation of microbiota composition is unclear. Here, we use a CD19-/- mouse model of antibody-deficiency
Approach to a patient with elevated serum alkaline phosphatase.
Siddique A and Kris V K
Clinics in Liver Disease, 16(2), 199-229 (2012)

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