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MAK309

Sigma-Aldrich

Bile Acid Assay Kit

sufficient for 100 fluorometric tests

NACRES:
NA.84

detection method

fluorometric

storage temp.

−20°C

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Bile Acid Assay Kit sufficient for 100 fluorometric tests

Sigma-Aldrich

MAK309

Bile Acid Assay Kit

Uric Acid Assay Kit sufficient for 100 colorimetric or fluorometric tests

Sigma-Aldrich

MAK077

Uric Acid Assay Kit

Sialic Acid Assay Kit sufficient for 100 colorimetric or fluorometric tests

Sigma-Aldrich

MAK314

Sialic Acid Assay Kit

detection method

fluorometric

detection method

colorimetric, fluorometric

detection method

colorimetric

detection method

colorimetric, fluorometric

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

Twelve different types of bile acids are typically found in mammals, among them two primary types are cholic acid and chenodeoxycholic acid. These can be dehydroxylated into secondary bile acids. Finally, these four can be conjugated to either taurine or glycine creating 8 different conjugated bile acids. Bile acid levels in feces, blood, urine, and bile can be used as markers for various diseases such as hyperlipidemia, cholestasis, gall stones, colon cancer, etc. Bile acids also exist as sulfate salt forms known as bile acid sulfates. Sulfation of bile acids increases their solubility and decreases intestinal absorption, thereby enhancing fecal and urinary excretion.

Application

Bile Acid Assay Kit has been used to quantify bile acid levels from plasma.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the quantitative determination of total bile acids and evaluation of drug effects on bile acid metabolism in serum, plasma, urine and other biological samples.

Principle

This Bile Acid Assay Kit provides a convenient fluorimetric means to measure total bile acids in biological samples. In the assay, 3–hydroxysteroid dehydrogenase reacts with all twelve bile acids, converting NAD to NADH, which reduces a probe to a highly fluorescent product. The resulting fluorescence intensity (γex = 530 nm/γem = 585 nm) is linear to the bile acid concentration in the sample.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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