Skip to Content
MilliporeSigma

Key Documents

View All Documentation

Skip To

MAK316

Heme Assay Kit

sufficient for 250 colorimetric tests

Synonym(s):

Heme Quantification Kit

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Size/SKUAvailabilityPrice
1 kit

Available to ship TODAYfromMILWAUKEE

$491.00

About This Item

NACRES:
NA.84
UNSPSC Code:
12161503

$491.00

Available to ship TODAYDetails

​
Request a Custom Order
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

detection method

colorimetric

relevant disease(s)

hematological disorder

storage temp.

2-8°C

General description

Heme is a member of the porphyrin family. It is synthesized in both mitochondria and cytoplasm, and is a key prosthetic group for various essential proteins such as hemoglobin, cytochromes, catalases and peroxidases. Free heme, which can be released from hemoglobin following hemolysis, is pro-inflammatory and contributes to iron-derived reactive oxygen species. Heme determination is widely practiced by researchers of various blood diseases.

Application

Suitable for the quantitative determination of heme and evaluation of drug effects on heme metabolism in a variety of biological samples such as blood, plasma, serum, urine, and heme carrying enzymes.

Biochem/physiol Actions

Heme assay is based on an aqueous alkaline solution method, in which the heme is converted into a uniform colored form, producing a colorimetric (400 nm) result, directly proportional to the heme concentration in the sample. The optimized formulation reduces interference and exhibits high sensitivity. One mg/dL heme equals 15.3M, 0.001%, or 10ppm. The kit has a linear detection range of 0.6–125M heme in a 96 well format.

Features and Benefits

Compatible with high-throughput handling systems.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
MAK115MAK311MAK165
Heme Assay Kit sufficient for 250 colorimetric tests

Sigma-Aldrich

MAK316

Heme Assay Kit

Hemoglobin Assay Kit sufficient for 250 colorimetric tests

Sigma-Aldrich

MAK115

Hemoglobin Assay Kit

Peroxide Assay Kit sufficient for 250 colorimetric tests

Sigma-Aldrich

MAK311

Peroxide Assay Kit

Fluorimetric Hydrogen Peroxide Assay Kit sufficient for 500 fluorometric tests (red fluorescence)

Sigma-Aldrich

MAK165

Fluorimetric Hydrogen Peroxide Assay Kit

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

fluorometric

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

relevant disease(s)

hematological disorder

relevant disease(s)

hematological disorder

relevant disease(s)

cardiovascular diseases; aging/geriatric diseases; neonatal diseases; pediatric diseases; orthopedic diseases; neurological disorders; pulmonary disorders

relevant disease(s)

cardiovascular diseases; aging/geriatric diseases; orthopedic diseases; pulmonary disorders; neurological disorders

pictograms

CorrosionEnvironment
GHS05,GHS09

signalword

Warning

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - Met. Corr. 1

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 2

Storage Class

12 - Non Combustible Liquids

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

1–9 of 9 Questions  

  1. Hello I am wondering would this sample work for fecal samples to quantify heme?

    1 answer

    1. There is no direct knowledge of using this product in stool samples. However, researchers have published studies utilizing this kit on fecal samples. For further details, refer to the following citation:

      Guéraud F, Buisson C, Promeyrat A, Naud N, Fouché E, Bézirard V, Dupuy J, Plaisancié P, Héliès-Toussaint C, Trouilh L, Martin JL, Jeuge S, Keuleyan E, Petit N, Aubry L, Théodorou V, Frémaux B, Olier M, Caderni G, Kostka T, Nassy G, Santé-Lhoutellier V, Pierre F. Effects of sodium nitrite reduction, removal or replacement on cured and cooked meat for microbiological growth, food safety, colon ecosystem, and colorectal carcinogenesis in Fischer 344 rats. NPJ Sci Food. 2023 Oct 7;7(1):53. doi: 10.1038/s41538-023-00228-9. PMID: 37805637; PMCID: PMC10560221.

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  3. How do you use this product on cell culture sample? How to homogenize the sample prior of heme measurement?

    1 answer

    1. Here is the recommended protocol for cell sample preparation:
      1. Homogenize cells with the heme reagent which contains NaOH and detergent which will effectively lyse the cells.
      2. Let incubate for 5 min for the color to develop, then centrifuge to pellet any debris.
      3. Transfer 250 µL of clear supernatant to a 96 well plate and measure the absorbance at 400 nm.

      Helpful?

  4. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  5. How does this kit work with cell culture sample? How do you homogenize the cells prior of measurement?

    1 answer

    1. This kit was validated for use with serum, plasma and whole blood. Cell lysates have not been analyzed with this kit and no specific guidance is provided for this type of sample preparation.

      Helpful?

  6. Could I use distilled water instead of ultrapure? Can I use this kit to measure how much plasma free hemoglobin I have in a plasma sample?

    1 answer

    1. Only ultra pure water should be used because it is filtered through a 0.22 μm filter which removes any bacteria that could interfere with the chemical reaction.

      Helpful?

  7. Does MAK316 work for mouse sera?

    1 answer

    1. MAK316 is expected to work for all species and should be compatible with mouse sera samples, as well as with other sample types such as blood, serum, plasma, and urine.

      Helpful?

  8. how is this kit used

    1 answer

    1. The kit is used according to the product protocol. Here is a link to that document:

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/386/102/mak316bul.pdf

      Helpful?

  9. お世話になります。臨床研究でヒトの血栓を回収してホルマリン固定しております。遊離ヘムを染めたいと考えておりますが　可能でしょうか？また、検体を送れば染めていただけるのでしょうか？

    1 answer

    1. This product is specifically tested for the quantitative determination of heme in various biological samples. Solid tissues are homogenized and lysed by the heme reagent which contains NaOH and a detergent. This would also apply to the clot sample resulting in the lysis of any intact RBCs. Given the principle of the kit, measuring only the free heme would not be possible. Currently, there is no product available to meet your requirements. Unfortunately, lab services are not offered for this particular application.

      Helpful?

Reviews

No rating value

Active Filters