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MAK320

Sigma-Aldrich

D-2-Hydroxyglutarate (D2HG) Assay Kit

sufficient for 200 fluorometric reactions

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usage

sufficient for 200 reactions (Fluorometric)

detection method

fluorometric

relevant disease(s)

cancer; neurological disorders

shipped in

wet ice

storage temp.

−20°C

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This Item
MAK062SAE0097MAK325
detection method

fluorometric

detection method

colorimetric

detection method

-

detection method

fluorometric

shipped in

wet ice

shipped in

-

shipped in

wet ice

shipped in

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

relevant disease(s)

cancer; neurological disorders

relevant disease(s)

cancer

relevant disease(s)

-

relevant disease(s)

obesity; cardiovascular diseases; cancer

General description

The level of D-2-Hydroxyglutarate (D2HG) is low in normal cells and tissues, but is significantly elevated in metabolic diseases, such as the rare autosomal disorder D2HG aciduria. D2HG is mildly elevated in other metabolic disorders including multiple acyl-CoA dehydrogenase deficiency, dihydrolipoyl dehydrogenase deficiency, pyruvate decarboxylase deficiency and pyruvate carboxylase deficiency, various cancers, and in neoplasms with mutations in the isocitrate dehydrogenase 1 (IDH1) and isocitrate dehydrogenase 2 (IDH2) genes. Detection of elevated D2HG is an important biomarker for early diagnosis, prognosis, monitoring and the development of therapeutic strategies against these diseases.

Features and Benefits

  • Highly sensitive and rapid enzymatic assay for the detection of D2HG levels in various biological fluids
  • Supportive calculator (Click here to download a calculator excel file) Analyze results based on your experimental data!
  • Quick instruction bench card - to assure your experimental success
  • More experiments in one kit - contains sufficient reagents for 200 tests
  • Detection time: only 30-60 minutes

Suitability

Suitable for the detection of D2HG levels in cells and tissues lysates, serum, urine, cultured cells and culture supernatants.

Principle

Developed in partnership with the German Cancer Research Center (DKFZ), the D2HG Assay Kit is a rapid and sensitive enzymatic assay for the detection of D2HG levels in various biological fluids: serum, urine, cell culture supernatants, and cell or tissue lysates. The assay, originally developed by Balss et al., 8 is based on the oxidation of D2HG to α-ketoglutarate (αKG) by the enzyme (D)-2-hydroxyglutarate dehydrogenase (HGDH) coupled to the reduction of NAD+ to NADH (see Figure 1). The amount of NADH formed is then quantitated by the diaphorase mediated reduction of resazurin to the fluorescent dye resorufin (λex = 540 nm/λem = 590 nm).

Legal Information

This product is sold under license from the German Cancer Research Center (DKFZ) and University Clinic of Heidelberg. Use of this product is covered by certain US and foreign patents, including US Pat. No. 9,487,815, EP Pat. No. 2 820 145 and foreign equivalents. Use of this product is for research purposes only.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


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Customers Also Viewed

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R A Chalmers et al.
Clinica chimica acta; international journal of clinical chemistry, 77(2), 117-124 (1977-06-01)
Detailed studies, using gas chromatography and mass spectrometric methods, of the urinary organic acids excreted by a patient with proven pyruvate decarboxylase deficiency are reported. In addition to the greatly-increased levels of lactate and pyruvate, marked elevation in the levels
Roberta Leonardi et al.
The Journal of biological chemistry, 287(18), 14615-14620 (2012-03-24)
Isocitrate dehydrogenase (IDH) is a reversible enzyme that catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (ICT) to α-ketoglutarate (αKG) and the NADPH/CO(2)-dependent reductive carboxylation of αKG to ICT. Reductive carboxylation by IDH1 was potently inhibited by NADP(+) and, to a
R A Chalmers et al.
Journal of inherited metabolic disease, 3(1), 11-15 (1980-01-01)
A patient with protein-losing gastroenteropathy and egg allergy has been shown to have a previously unrecognized organic aciduria, D-2-hydroxyglutaric aciduria. The observations made are consistent with an inherited metabolic disorder in the catabolism of 5-aminolaevulinate possibly due to deficient activity
Julie-Aurore Losman et al.
Genes & development, 27(8), 836-852 (2013-05-01)
Mutations in metabolic enzymes, including isocitrate dehydrogenase 1 (IDH1) and IDH2, in cancer strongly implicate altered metabolism in tumorigenesis. IDH1 and IDH2 catalyze the interconversion of isocitrate and 2-oxoglutarate (2OG). 2OG is a TCA cycle intermediate and an essential cofactor
Eduard A Struys et al.
American journal of human genetics, 76(2), 358-360 (2004-12-21)
d-2-hydroxyglutaric aciduria is a neurometabolic disorder with both a mild and a severe phenotype and with unknown etiology. Recently, a novel enzyme, d-2-hydroxyglutarate dehydrogenase, which converts d-2-hydroxyglutarate into 2-ketoglutarate, and its gene were identified. In the genes of two unrelated

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