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MAK341

Sigma-Aldrich

Deproteinizing Sample Preparation Kit

sufficient for 200 assays

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Synonym(s):
Deproteinization Kit
UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 200 assays

application(s)

sample preservation

General description

The presence of protein and various enzyme activities frequently interferes with the analysis of small molecules in biological samples. Many bioassays require removal of proteins from samples prior to analysis. Among the deproteinization protocols developed over the last half century, perchloric acid (PCA) precipitation has been extensively used in many different sample preparation procedures, since not only does it remove most of the protein present but it also functions to stabilize many of the small molecule analytes. PCA deproteinization methods have been successfully used in the preparation samples prior to quantitation of an array of small molecules, including glycogen, ATP, cAMP, glutathione, antioxidants, etc.

Application


  • Protein Removal for LC-MS/MS in Clinical Biochemistry: The deproteinizing sample preparation kit facilitated the removal of proteins from whole blood using paramagnetic micro-particles, enhancing the accuracy of LC-MS/MS analyses in clinical settings (Van den Bossche et al., 2014).

  • Efficient Sample Prep for Metabolomics Research: The kit was applied to deproteinize serum samples using magnetic micro-particles, proving essential for reliable LC-MS/MS analysis in clinical biochemistry, particularly for metabolomics research (König et al., 2013).

Suitability

Suitable for deproteinizing various biological samples

Principle

The Deproteinizing Sample Preparation Kit utilizes a PCA precipitation method, providing a unique tool to prepare samples for the analysis of a variety of small molecules

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Met. Corr. 1 - Ox. Liq. 2 - Skin Corr. 1A - STOT RE 2

target_organs

Thyroid

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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