MAK390

Alpha Galactosidase (α-Gal) Activity Assay Kit (Fluorometric)

sufficient for 100 fluorometric tests

Properties

• Quality Level: 100
• detection method: fluorometric
• storage temp.: −20°C

Description

General description

Alpha-Galactosidase (α-Gal) hydrolyzes alpha-galactosyl moieties found in glycolipids and glycoproteins. In mammals, a-Gal hydrolyzes poly- and oligosaccharides commonly found in dietary sources that are difficult to digest. Therefore, α-Gal is used in dietary supplements that help to reduce the production of intestinal gases due to consumption of certain foods. It is known total α-Gal activity is due to two major isozymes with unique, yet different thermostability profiles. Alpha-Galactosidase A, is thermolabile and represents approximately 90% of total α-Gal activity. Fabry Disease, a lysosomal disease disorder, is characterized by mutations in alpha-Galactosidase A. These mutations cause abnormal accumulation of glycosphingolipids in lysosomes.

Packaging

1 kit

Features and Benefits

The Alpha Galactosidase (α-Gal) Activity Assay Kit:

• Detection limit: < 0.1μU.
• Specific: does not detect beta galactosidase activity.

Suitability

The kit is suitable for the detection of α-Galactosidase activity in tissue homogenates (kidney, etc.), cell lysates (U937, etc.) and biological fluids (saliva, etc.).

Principle

The Alpha Galactosidase (α-Gal) Activity Assay Kit provides a simple, rapid way to monitor total α-Gal activity in a wide variety of biological samples. In this kit, α-Gal cleaves a synthetic specific substrate releasing a fluorophore, which can be easily quantified (λ_ex = 360 nm/λ_em = 445 nm). The assay is specific (beta galactosidase activity is not detected), sensitive and can detect as low as 0.1 μU of α-Galactosidase activity.

Safety Information

• Storage Class Code: 10 - Combustible liquids