MAK441
Kinase Assay Kit
Sufficient for 400 fluorometric tests
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Product Name
Kinase Assay Kit, Sufficient for 400 fluorometric tests
application(s)
pharmaceutical
detection method
fluorometric
storage temp.
−20°C
Quality Level
Related Categories
1 of 4
This Item | SAB1300475 | MAK173 | MAK116 |
|---|---|---|---|
| Quality Level 100 | Quality Level 200 | Quality Level - | Quality Level - |
| detection method fluorometric | detection method - | detection method fluorometric | detection method colorimetric |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| application(s) pharmaceutical | application(s) - | application(s) - | application(s) - |
Application
The Kinase Assay Kit may be used for:
- Cancer Research
- Cell Signaling Research
- Drug Discovery
The kit is suitable for the determination of protein kinase activity and for high-throughput screening of kinase inhibitors.
Biochem/physiol Actions
The Kinase Assay Kit provides a simple and rapid method for assaying kinase activity and high-throughput screening for kinase inhibitors. This homogeneous microplate-based assay involves incubating the kinase with a single working reagent, in which ADP is enzymatically converted to ATP and pyruvate, which is quantified using a fluorometric (Ex = λ530 nm/λEm = 590 nm) assay method.
Features and Benefits
- High Sensitivity and Accuracy: Achieve quantification of kinase activity, detecting as low as 0.01 U/L with high sensitivity and accuracy.
- Streamlined Procedure: Effortlessly conduct the assay with a simplified process, adding just a single working reagent. Complete the assay in a quick 10-minute reaction, optimizing efficiency and saving valuable time.
- High-Throughput Inhibitor Screening: Screen for kinase inhibitors effectively with a minimal 5 μL test compound requirement, enhancing efficiency in high-throughput settings.
General description
Kinases, alternatively referred to as phosphotransferases, form an extensive family of enzymes responsible for transferring phosphate groups from the high-energy donor molecule ATP to their designated substrates. This family of enzymes is renowned for its role in regulating a myriad of cellular processes, with the protein kinases constituting the largest subgroup. In humans, 518 distinct kinases have been identified, and a substantial portion of human proteins, up to 30%, undergo modification by these kinases. The considerable diversity and pivotal role of kinases in cellular signaling position them as promising targets for drug development, biochemical and biomedical research.
Other Notes
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Storage Class
10 - Combustible liquids
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