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MAK471

Sigma-Aldrich

Urea Assay Kit III

new

sufficient for 100 colorimetric tests

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Quality Level

application(s)

food and beverages

detection method

colorimetric

relevant disease(s)

diabetes; renal disease; gastrointestinal diseases; endocrinological disorders; genitourinary disorders; cardiovascular diseases; orthopedic diseases

shipped in

wet ice

storage temp.

−20°C

Related Categories

General description

Urea is the major end product of protein catabolism in animals. Urea is primarily produced in the liver and secreted by the kidneys. It is the primary vehicle for removal of toxic ammonia from the body. Urea determination is useful in assessing kidney function. In general, increased urea levels are associated with nephritis, renal ischemia, urinary tract obstruction, and certain extrarenal diseases (e.g., congestive heart failure, liver diseases, and diabetes). Decreased levels often indicate acute hepatic insufficiency but may also result from over vigorous parenteral fluid therapy.

Features and Benefits

  • The linear detection range of the kit is 50 to 1000 µM urea in the 96-well plate assay
  • Compatible with high-throughput handling systems.
  • Simple procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37 °C heater is needed.

Suitability

The kit is suitable for detection of urea in biological samples such as plasma, serum, urine, bronchoalveolar lavage (BAL), and food/beverage samples such as milk.

Principle

The Urea Assay Kit III is designed to directly measure urea in biological samples. In this assay, urease converts urea to ammonia and carbon dioxide. NADH is then converted to NAD+ in the presence of ammonia, α-ketoglutarate, and glutamate dehydrogenase. The decrease in optical density at 340 nm is directly proportional to the urea concentration in the sample.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


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