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MAK532
Nitric Oxide Synthase (NOS) Assay Kit
sufficient for 100 colorimetric tests
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
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Quality Level
application(s)
pharmaceutical
detection method
colorimetric
relevant disease(s)
cardiovascular diseases; immunological diseases; aging/geriatric diseases; cancer
shipped in
wet ice
storage temp.
−20°C
Related Categories
General description
Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO, an oxidation product of arginine by nitric oxide synthase (NOS), is involved in host defense and development, activation of regulatory proteins and direct covalent interaction with functional biomolecules.
Application
The kit is suitable for the determination of nitric oxide synthase activity in biological samples, as well as for studying the effects of drugs on nitric oxide synthase activity.
Biochem/physiol Actions
The Nitric Oxide Synthase (NOS) Assay Kit nvolves two steps: a NOS reaction step during which NO is produced followed by an NO detection step. Since the NO generated by NOS is rapidly oxidized to nitrite and nitrate, the NO production is measured following reduction of nitrate to nitrite using an improved Griess method. The procedure is simple and the time required for sample pretreatment and assay is reduced to as short as 40 minutes.
Features and Benefits
- Linear detection range: 0.25 - 25 U/L
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Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A
Storage Class
8A - Combustible corrosive hazardous materials
wgk_germany
WGK 3
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Instructions
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The protocol is included in the product datasheet, which may be accessed in the DOCUMENTATION section. Please see the link below to review:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/162/023/mak532pis-ms.pdfHelpful?
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